Reggie/flotillin proteins are organized into stable tetramers in membrane microdomains

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119_Reggie_flotillin_proteins_are_organized.pdf
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2007
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Biochemical Journal. 2007, 403(2), pp. 313-322. ISSN 0006-2936. eISSN 1470-8728
Zusammenfassung

Reggie-1 and -2 proteins (flotillin-2 and -1 respectively) form their own type of non-caveolar membrane microdomains, which are involved in important cellular processes such as T-cell activation, phagocytosis and signallingmediated by the cellular prion protein and insulin; this is consistent with the notion that reggie microdomains promote protein assemblies and signalling. While it is generally known that membrane microdomains contain large multiprotein assemblies, the exact organization of reggiemicrodomains remains elusive. Using chemical cross-linking approaches, we have demonstrated that reggie complexes are composed of homoand hetero-tetramers of reggie-1 and -2. Moreover, native reggie oligomers are indeed quite stable, since non-cross-linked tetramers are resistant to 8 M urea treatment. We also show that oligomerization requires the C-terminal but not the N-terminal halves of reggie-1 and -2. Using deletion constructs, we analysed the functional relevance of the three predicted coiled-coil stretches present in the C-terminus of reggie-1. We confirmed experimentally that reggie-1 tetramerization is dependent on the presence of coiled-coil 2 and, partially, of coiled-coil 1. Furthermore, since depletion of reggie-1 by siRNA (small interfering RNA) silencing induces proteasomal degradation of reggie-2, we conclude that the protein stability of reggie-2 depends on the presence of reggie-1. Our data indicate that the basic structural units of reggie microdomains are reggie homo- and hetero-tetramers, which are dependent on the presence of reggie-1.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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cross-linking, flotillin, lipid raft, membrane microdomain, oligomerization, reggie
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ISO 690SOLIS PADILLA, Gonzalo, Maja HOEGG, Christina MUNDERLOH, Yvonne SCHROCK, Edward MÁLAGA-TRILLO, Eric RIVERA-MILLA, Claudia STÜRMER, 2007. Reggie/flotillin proteins are organized into stable tetramers in membrane microdomains. In: Biochemical Journal. 2007, 403(2), pp. 313-322. ISSN 0006-2936. eISSN 1470-8728
BibTex
@article{SolisPadilla2007Reggi-7279,
  year={2007},
  title={Reggie/flotillin proteins are organized into stable tetramers in membrane microdomains},
  number={2},
  volume={403},
  issn={0006-2936},
  journal={Biochemical Journal},
  pages={313--322},
  author={Solis Padilla, Gonzalo and Hoegg, Maja and Munderloh, Christina and Schrock, Yvonne and Málaga-Trillo, Edward and Rivera-Milla, Eric and Stürmer, Claudia}
}
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