Publikation:

Enabling technologies of genomic-scale sequence enrichment for targeted high-throughput sequencing

Lade...
Vorschaubild

Dateien

Summerer.pdf
Summerer.pdfGröße: 19.2 MBDownloads: 472

Datum

2009

Autor:innen

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Genomics. 2009, 94(6), pp. 363-368. ISSN 0888-7543. eISSN 1089-8646. Available under: doi: 10.1016/j.ygeno.2009.08.012

Zusammenfassung

Next-generation sequencing has still not reached its full potential due to the technical inability of effectively targeting desired genomic regions of interest. Once available, methods adressing this bottleneck will dramatically reduce cost and enable the efficient analysis of complex samples. Recently, a number of possible approaches for genomic-scale sequence enrichment have been reported using different strategies. All methods basically rely on sequence-specific nucleic acid hybridization, however, they differ in several aspects such as the use of solid phase versus solution phase hybridization, probe design and overall workflows with implications for automation. Overall, several key challenges of genome-wide sequence enrichment have become clear after these studies that remain to be overcome. We summarize the different technologies and highlight individual characteristics related to general potential and different suitabilities for specific applications.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690SUMMERER, Daniel, 2009. Enabling technologies of genomic-scale sequence enrichment for targeted high-throughput sequencing. In: Genomics. 2009, 94(6), pp. 363-368. ISSN 0888-7543. eISSN 1089-8646. Available under: doi: 10.1016/j.ygeno.2009.08.012
BibTex
@article{Summerer2009-12Enabl-402,
  year={2009},
  doi={10.1016/j.ygeno.2009.08.012},
  title={Enabling technologies of genomic-scale sequence enrichment for targeted high-throughput sequencing},
  number={6},
  volume={94},
  issn={0888-7543},
  journal={Genomics},
  pages={363--368},
  author={Summerer, Daniel}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/402">
    <dc:format>application/pdf</dc:format>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-07-04T08:30:29Z</dcterms:available>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/402/1/Summerer.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-07-04T08:30:29Z</dc:date>
    <dc:language>eng</dc:language>
    <dcterms:bibliographicCitation>First publ. in: Genomics 94 (2009), 6, pp. 363-368</dcterms:bibliographicCitation>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/402"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dcterms:title>Enabling technologies of genomic-scale sequence enrichment for targeted high-throughput sequencing</dcterms:title>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Summerer, Daniel</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>2009-12</dcterms:issued>
    <dcterms:abstract xml:lang="eng">Next-generation sequencing has still not reached its full potential due to the technical inability of effectively targeting desired genomic regions of interest. Once available, methods adressing this bottleneck will dramatically reduce cost and enable the efficient analysis of complex samples. Recently, a number of possible approaches for genomic-scale sequence enrichment have been reported using different strategies. All methods basically rely on sequence-specific nucleic acid hybridization, however, they differ in several aspects such as the use of solid phase versus solution phase hybridization, probe design and overall workflows with implications for automation. Overall, several key challenges of genome-wide sequence enrichment have become clear after these studies that remain to be overcome. We summarize the different technologies and highlight individual characteristics related to general potential and different suitabilities for specific applications.</dcterms:abstract>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/402/1/Summerer.pdf"/>
    <dc:contributor>Summerer, Daniel</dc:contributor>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen