Differential distribution of calcium stores in Paramecium cells : occurrence of a subplasmalemmal store with a calsequestrin-like protein

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1997
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Habermann, Anja
Klauke, Norbert
Majoul, Irina
Söling, Hans-Dieter
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We have analyzed in Paramecium cells the occurrence and intracellular distribution of the high capacity/low affinity calcium-binding proteins, calsequestrin (CS) and calreticulin (CR) using antibodies against CS from rat skeletal muscle and against CR from rat liver, respectively. As revealed by Western blots, a CS-like protein isolated by affinity chromatography from Paramecium cells comigrated with CS isolated from rat skeletal muscle. The immunoreactivity of this 53 kDa protein band was blocked when the antibodies had been preadsorbed with purified rat CS. A band of identical molecular size was shown to bind 45Ca in overlays. By immunofluorescence and immunogold labeling this CS-like protein was localized selectively to the extended subplasmalemmal calcium stores, the "alveolar sacs", which cover almost the entire cell surface. Concomitantly the 53 kDa 45Ca-binding band became increasingly intense in overlays as we increasingly enriched alveolar sacs. Antibodies against rat CR react with a 61 kDa band but do not cross-react with CS-like protein in Paramecium. These antibodies selectively stained intracellular reticular structures, identified bona fide as endoplasmic reticulum.

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570 Biowissenschaften, Biologie
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Calcium, calreticulin, calsequestrin, exocytosis, Paramecium
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ISO 690PLATTNER, Helmut, Anja HABERMANN, Roland KISSMEHL, Norbert KLAUKE, Irina MAJOUL, Hans-Dieter SÖLING, 1997. Differential distribution of calcium stores in Paramecium cells : occurrence of a subplasmalemmal store with a calsequestrin-like protein. In: European Journal of Cell Biology. 1997, 72, pp. 297-306
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@article{Plattner1997Diffe-6592,
  year={1997},
  title={Differential distribution of calcium stores in Paramecium cells : occurrence of a subplasmalemmal store with a calsequestrin-like protein},
  volume={72},
  journal={European Journal of Cell Biology},
  pages={297--306},
  author={Plattner, Helmut and Habermann, Anja and Kissmehl, Roland and Klauke, Norbert and Majoul, Irina and Söling, Hans-Dieter}
}
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