Gene Electrotransfer : a Mechanistic Perspective

Rosazza, Christelle; Meglic, Sasa Haberl; Zumbusch, Andreas; Rols, Marie-Pierre; Miklavcic, Damijan

Gene Electrotransfer : a Mechanistic Perspective

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Date

2016

Authors

Rosazza, Christelle

Meglic, Sasa Haberl

Zumbusch, Andreas

Rols, Marie-Pierre

Miklavcic, Damijan

DOI (citable link)

10.2174/1566523216666160331130040

Collections

Chemie

Publication type

Journal article

Publication status

Published

Published in

Current Gene Therapy ; 16 (2016), 2. - pp. 98-129. - ISSN 1566-5232. - eISSN 1875-5631

Abstract

Gene electrotransfer is a powerful method of DNA delivery offering several medical applications, among the most promising of which are DNA vaccination and gene therapy for cancer treatment. Electroporation entails the application of electric fields to cells which then experience a local and transient change of membrane permeability. Although gene electrotransfer has been extensively studied in in vitro and in vivo environments, the mechanisms by which DNA enters and navigates through cells are not fully understood. Here we present a comprehensive review of the body of knowledge concerning gene electrotransfer that has been accumulated over the last three decades. For that purpose, after briefly reviewing the medical applications that gene electrotransfer can provide, we outline membrane electropermeabilization, a key process for the delivery of DNA and smaller molecules. Since gene electrotransfer is a multipart process, we proceed our review in describing step by step our current understanding, with particular emphasis on DNA internalization and intracellular trafficking. Finally, we turn our attention to in vivo testing and methodology for gene electrotransfer. - See more at: http://www.eurekaselect.com/140801/article#sthash.ZcmWXtQH.dpuf

Subject (DDC)

540 Chemistry

Cite This

ISO 690

ROSAZZA, Christelle, Sasa Haberl MEGLIC, Andreas ZUMBUSCH, Marie-Pierre ROLS, Damijan MIKLAVCIC, 2016. Gene Electrotransfer : a Mechanistic Perspective. In: Current Gene Therapy. 16(2), pp. 98-129. ISSN 1566-5232. eISSN 1875-5631. Available under: doi: 10.2174/1566523216666160331130040

BibTex

@article{Rosazza2016Elect-34528,
  year={2016},
  doi={10.2174/1566523216666160331130040},
  title={Gene Electrotransfer : a Mechanistic Perspective},
  number={2},
  volume={16},
  issn={1566-5232},
  journal={Current Gene Therapy},
  pages={98--129},
  author={Rosazza, Christelle and Meglic, Sasa Haberl and Zumbusch, Andreas and Rols, Marie-Pierre and Miklavcic, Damijan}
}

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Bibliography of Konstanz

Yes