Analysing size variation during light-starvation response of nutritionally diverse chrysophytes with a Coulter counter

Rottberger, Julia; Gruber, Ansgar; Kroth, Peter G.

Analysing size variation during light-starvation response of nutritionally diverse chrysophytes with a Coulter counter

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2013

Projekt

Organismic Interactions in Aquatic Ecosystems under Changing Conditions TP C2

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Erschienen in

Algological Studies ; 141 (2013), 1. - S. 37-51. - ISSN 1864-1318

Zusammenfassung

Mixotrophy is a combination of autotrophic and heterotrophic nutrition. The regulation of the nutritional mode in mixotrophic algae is not yet fully understood. Changes in the light regime are likely to affect cell size of autotrophic, mixotrophic and heterotrophic chrysophytes to a varying extend. Here we demonstrate that an automatic particle analyser (Coulter counter) is a helpful tool not only for cell counts, but also for the detection of changes in mean cell size, total cell bio-volume or size distribution of chrysophyte cultures. The method is time saving and therefore allows large comparative studies, however, the method tends to underestimate the absolute size compared to microscopic measurements. The limitations of automated particle analysis opposed to traditional microscopical measurements are discussed exemplarily for Dinobryon divergens.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

ROTTBERGER, Julia, Ansgar GRUBER, Peter G. KROTH, 2013. Analysing size variation during light-starvation response of nutritionally diverse chrysophytes with a Coulter counter. In: Algological Studies. 141(1), pp. 37-51. ISSN 1864-1318. Available under: doi: 10.1127/1864-1318/2013/0105

BibTex

@article{Rottberger2013Analy-21966,
  year={2013},
  doi={10.1127/1864-1318/2013/0105},
  title={Analysing size variation during light-starvation response of nutritionally diverse chrysophytes with a Coulter counter},
  number={1},
  volume={141},
  issn={1864-1318},
  journal={Algological Studies},
  pages={37--51},
  author={Rottberger, Julia and Gruber, Ansgar and Kroth, Peter G.}
}

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Universitätsbibliographie

Ja