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RpoS Acts as a Global Repressor of Virulence Gene Expression in Escherichia coli O104:H4 and Enteroaggregative E coli

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2024

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Berger, Petya
Dumevi, Rexford M.
Berger, Michael
Hastor, Ines
Treffon, Janina
Kehl, Alexander
Scherff, Natalie
Dobrindt, Ulrich
Mellmann, Alexander

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https://doi.org/10.1093/infdis/jiae151
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The Journal of Infectious Diseases. Oxford University Press (OUP). 2024, 230(4), S. 840-851. ISSN 0022-1899. eISSN 1537-6613. Verfügbar unter: doi: 10.1093/infdis/jiae151

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In 2011, in Germany, Escherichia coli O104:H4 caused the enterohemorrhagic E coli (EHEC) outbreak with the highest incidence rate of hemolytic uremic syndrome. This pathogen carries an exceptionally potent combination of EHEC- and enteroaggregative E coli (EAEC)–specific virulence factors. Here, we identified an E coli O104:H4 isolate that carried a single-nucleotide polymorphism (SNP) in the start codon (ATG > ATA) of rpoS, encoding the alternative sigma factor S. The rpoS ATG > ATA SNP was associated with enhanced EAEC-specific virulence gene expression. Deletion of rpoS in E coli O104:H4 Δstx2 and typical EAEC resulted in a similar effect. Both rpoS ATG > ATA and ΔrpoS strains exhibited stronger virulence-related phenotypes in comparison to wild type. Using promoter-reporter gene fusions, we demonstrated that wild-type RpoS repressed aggR, encoding the main regulator of EAEC virulence. In summary, our work demonstrates that RpoS acts as a global repressor of E coli O104:H4 virulence, primarily through an AggR-dependent mechanism.

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570 Biowissenschaften, Biologie

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ISO 690BERGER, Petya, Rexford M. DUMEVI, Michael BERGER, Ines HASTOR, Janina TREFFON, Ian U. KOUZEL, Alexander KEHL, Natalie SCHERFF, Ulrich DOBRINDT, Alexander MELLMANN, 2024. RpoS Acts as a Global Repressor of Virulence Gene Expression in Escherichia coli O104:H4 and Enteroaggregative E coli. In: The Journal of Infectious Diseases. Oxford University Press (OUP). 2024, 230(4), S. 840-851. ISSN 0022-1899. eISSN 1537-6613. Verfügbar unter: doi: 10.1093/infdis/jiae151
BibTex
@article{Berger2024Globa-69953,
  year={2024},
  doi={10.1093/infdis/jiae151},
  title={RpoS Acts as a Global Repressor of Virulence Gene Expression in Escherichia coli O104:H4 and Enteroaggregative E coli},
  number={4},
  volume={230},
  issn={0022-1899},
  journal={The Journal of Infectious Diseases},
  pages={840--851},
  author={Berger, Petya and Dumevi, Rexford M. and Berger, Michael and Hastor, Ines and Treffon, Janina and Kouzel, Ian U. and Kehl, Alexander and Scherff, Natalie and Dobrindt, Ulrich and Mellmann, Alexander}
}
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