Quantification of bacterial invasion into adherent cells by flow cytometry

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2006
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Schmitter, Tim
Neske, Florian
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Zusammenfassung

Quantification of invasive, intracellular bacteria is critical in many areas of cellular microbiology and immunology. We describe a novel and fast approach to determine invasion of bacterial pathogens in adherent cell types such as epithelial cells or fibroblasts based on flow cytometry. Using the CEACAM-mediated uptake of Opa-expressing Neisseria gonorrhoeae as a well-characterized model of bacterial invasion, we demonstrate that the flow cytometry-based method yields results comparable to a standard antibiotic protection assay. Furthermore, the quantification of intracellular bacteria by the novel approach is not biased by intracellular killing of the microbes and correctly discriminates between cell-associated extracellular and bona fide intracellular bacteria. As flow cytometry-based quantification is also applicable to other pathogen-host interactions such as the integrin-mediated intemalization of Staphylococcus aureus, this approach provides a fast and convenient alternative for the quantification of bacterial uptake and should be particularly useful in elucidating the molecular mechanisms of pathogen-triggered host cell invasion.

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570 Biowissenschaften, Biologie
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Bacterial invasion, Intracellular bacteria, Fluorescence labelling, Flow cytometry, Neisseria gonorrhoeae, Staphylococcus aureus
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ISO 690PILS, Stefan, Tim SCHMITTER, Florian NESKE, Christof R. HAUCK, 2006. Quantification of bacterial invasion into adherent cells by flow cytometry. In: Journal of Microbiological Methods. 2006, 65(2), pp. 301-310. ISSN 0167-7012. eISSN 1872-8359
BibTex
@article{Pils2006Quant-8132,
  year={2006},
  title={Quantification of bacterial invasion into adherent cells by flow cytometry},
  number={2},
  volume={65},
  issn={0167-7012},
  journal={Journal of Microbiological Methods},
  pages={301--310},
  author={Pils, Stefan and Schmitter, Tim and Neske, Florian and Hauck, Christof R.}
}
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