@article{Pils2006Quant-8132,
  year={2006},
  title={Quantification of bacterial invasion into adherent cells by flow cytometry},
  number={2},
  volume={65},
  issn={0167-7012},
  journal={Journal of Microbiological Methods},
  pages={301--310},
  author={Pils, Stefan and Schmitter, Tim and Neske, Florian and Hauck, Christof R.}
}

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    <dc:creator>Pils, Stefan</dc:creator>
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    <dc:contributor>Hauck, Christof R.</dc:contributor>
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    <dc:contributor>Schmitter, Tim</dc:contributor>
    <dc:creator>Schmitter, Tim</dc:creator>
    <dc:contributor>Pils, Stefan</dc:contributor>
    <dc:creator>Neske, Florian</dc:creator>
    <dcterms:title>Quantification of bacterial invasion into adherent cells by flow cytometry</dcterms:title>
    <dc:contributor>Neske, Florian</dc:contributor>
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    <dcterms:abstract xml:lang="deu">Quantification of invasive, intracellular bacteria is critical in many areas of cellular microbiology and immunology. We describe a novel and fast approach to determine invasion of bacterial pathogens in adherent cell types such as epithelial cells or fibroblasts based on flow cytometry. Using the CEACAM-mediated uptake of Opa-expressing Neisseria gonorrhoeae as a well-characterized model of bacterial invasion, we demonstrate that the flow cytometry-based method yields results comparable to a standard antibiotic protection assay. Furthermore, the quantification of intracellular bacteria by the novel approach is not biased by intracellular killing of the microbes and correctly discriminates between cell-associated extracellular and bona fide intracellular bacteria. As flow cytometry-based quantification is also applicable to other pathogen-host interactions such as the integrin-mediated intemalization of Staphylococcus aureus, this approach provides a fast and convenient alternative for the quantification of bacterial uptake and should be particularly useful in elucidating the molecular mechanisms of pathogen-triggered host cell invasion.</dcterms:abstract>
    <dcterms:issued>2006</dcterms:issued>
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    <dcterms:bibliographicCitation>First publ. in: Journal of Microbiological Methods 65 (2006), 2, pp. 301 310</dcterms:bibliographicCitation>
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