The ubiquitin-like modifier FAT10 inhibits retinal PDE6 activity and mediates its proteasomal degradation

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2020
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Sacristan-Reviriego, Almudena
van der Spuy, Jacqueline
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The Journal of Biological Chemistry (JBC). American Society for Biochemistry and Molecular Biology. 2020, 295(42), pp. 14402-14418. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.RA120.013873
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The retina-specific chaperone AIPL1 is essential for the correct assembly of phosphodiesterase 6 (PDE6), which is a pivotaleffector enzyme for phototransduction and vision because it hydrolyzes cGMP. AIPL1 interacts with the cytokine-inducible ubiquitin-likemodifier FAT10 that gets covalently conjugated to hundreds of proteins and targets its conjugation substrates for proteasomaldegradation, but whether FAT10 affects PDE6 function or turnover is unknown. Here, we show that FAT10 mRNA is expressed inhuman retina and identify rod PDE6 as a retina-specific substrate of FAT10 conjugation. We found that AIPL1 stabilizes theFAT10 monomer as well as the PDE6-FAT10 conjugate. Additionally, we elucidated the functional consequences of PDE6 FAT10ylation.On the one hand, we demonstrate that FAT10 targets PDE6 for proteasomal degradation by formation of a covalent isopeptidelinkage. On the other hand, FAT10 inhibits PDE6 cGMP hydrolyzing activity by non-covalently interacting with the PDE6 GAFaand catalytic domains. Therefore, FAT10 may contribute to loss of PDE6 and, as a consequence, degeneration of retinal cellsin eye diseases linked to inflammation and inherited blindness causing mutations in AIPL1.

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ISO 690BOEHM, Annika N., Johanna BIALAS, Nicola CATONE, Almudena SACRISTAN-REVIRIEGO, Jacqueline VAN DER SPUY, Marcus GRÖTTRUP, Annette AICHEM, 2020. The ubiquitin-like modifier FAT10 inhibits retinal PDE6 activity and mediates its proteasomal degradation. In: The Journal of Biological Chemistry (JBC). American Society for Biochemistry and Molecular Biology. 2020, 295(42), pp. 14402-14418. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.RA120.013873
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@article{Boehm2020-10-16ubiqu-50693,
  year={2020},
  doi={10.1074/jbc.RA120.013873},
  title={The ubiquitin-like modifier FAT10 inhibits retinal PDE6 activity and mediates its proteasomal degradation},
  number={42},
  volume={295},
  issn={0021-9258},
  journal={The  Journal of Biological Chemistry (JBC)},
  pages={14402--14418},
  author={Boehm, Annika N. and Bialas, Johanna and Catone, Nicola and Sacristan-Reviriego, Almudena and van der Spuy, Jacqueline and Gröttrup, Marcus and Aichem, Annette}
}
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    <dcterms:abstract xml:lang="eng">The retina-specific chaperone AIPL1 is essential for the correct assembly of phosphodiesterase 6 (PDE6), which is a pivotaleffector enzyme for phototransduction and vision because it hydrolyzes cGMP. AIPL1 interacts with the cytokine-inducible ubiquitin-likemodifier FAT10 that gets covalently conjugated to hundreds of proteins and targets its conjugation substrates for proteasomaldegradation, but whether FAT10 affects PDE6 function or turnover is unknown. Here, we show that FAT10 mRNA is expressed inhuman retina and identify rod PDE6 as a retina-specific substrate of FAT10 conjugation. We found that AIPL1 stabilizes theFAT10 monomer as well as the PDE6-FAT10 conjugate. Additionally, we elucidated the functional consequences of PDE6 FAT10ylation.On the one hand, we demonstrate that FAT10 targets PDE6 for proteasomal degradation by formation of a covalent isopeptidelinkage. On the other hand, FAT10 inhibits PDE6 cGMP hydrolyzing activity by non-covalently interacting with the PDE6 GAFaand catalytic domains. Therefore, FAT10 may contribute to loss of PDE6 and, as a consequence, degeneration of retinal cellsin eye diseases linked to inflammation and inherited blindness causing mutations in AIPL1.</dcterms:abstract>
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