Extracellular Allosteric Na+ Binding to the Na+,K+-ATPase in Cardiac Myocytes

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2013
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Garcia, Alvaro
Fry, Natasha A.S.
Karimi, Keyvan
Liu, Chia-chi
Rasmussen, Helge
Clarke, Ronald
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Biophysical Journal. 2013, 105(12), pp. 2695-2705. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2013.11.004
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Whole-cell patch-clamp measurements of the current, Ip, produced by the Na+,K+-ATPase across the plasma membrane of rabbit cardiac myocytes show an increase in Ip over the extracellular Na+ concentration range 0–50 mM. This is not predicted by the classical Albers-Post scheme of the Na+,K+-ATPase mechanism, where extracellular Na+ should act as a competitive inhibitor of extracellular K+ binding, which is necessary for the stimulation of enzyme dephosphorylation and the pumping of K+ ions into the cytoplasm. The increase in Ip is consistent with Na+ binding to an extracellular allosteric site, independent of the ion transport sites, and an increase in turnover via an acceleration of the rate-determining release of K+ to the cytoplasm, E2(K+)2 → E1 + 2K+. At normal physiological concentrations of extracellular Na+ of 140 mM, it is to be expected that binding of Na+ to the allosteric site would be nearly saturated. Its purpose would seem to be simply to optimize the enzyme’s ion pumping rate under its normal physiological conditions. Based on published crystal structures, a possible location of the allosteric site is within a cleft between the α- and β-subunits of the enzyme

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ISO 690GARCIA, Alvaro, Natasha A.S. FRY, Keyvan KARIMI, Chia-chi LIU, Hans-Jürgen APELL, Helge RASMUSSEN, Ronald CLARKE, 2013. Extracellular Allosteric Na+ Binding to the Na+,K+-ATPase in Cardiac Myocytes. In: Biophysical Journal. 2013, 105(12), pp. 2695-2705. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2013.11.004
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@article{Garcia2013-12-17Extra-25574,
  year={2013},
  doi={10.1016/j.bpj.2013.11.004},
  title={Extracellular Allosteric Na<sup>+</sup> Binding to the Na<sup>+</sup>,K<sup>+</sup>-ATPase in Cardiac Myocytes},
  number={12},
  volume={105},
  issn={0006-3495},
  journal={Biophysical Journal},
  pages={2695--2705},
  author={Garcia, Alvaro and Fry, Natasha A.S. and Karimi, Keyvan and Liu, Chia-chi and Apell, Hans-Jürgen and Rasmussen, Helge and Clarke, Ronald}
}
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