Isolation, Characterization, and Genome Sequence Analysis of a Novel Lytic Phage, Xoo-sp15 Infecting Xanthomonas oryzae pv. oryzae

Nazir, Amina; Dong, Zhaoxia; Liu, Jin; Tahir, Rana Adnan; Ashraf, Neelma; Qing, Hong; Peng, Donghai; Tong, Yigang

Isolation, Characterization, and Genome Sequence Analysis of a Novel Lytic Phage, Xoo-sp15 Infecting Xanthomonas oryzae pv. oryzae

Dateien

Nazir_2-kpx7stcu63zl0.pdfGröße: 949.03 KBDownloads: 184

Datum

2021

Autor:innen

Nazir, Amina

Dong, Zhaoxia

Liu, Jin

Tahir, Rana Adnan

Ashraf, Neelma

Qing, Hong

Peng, Donghai

Tong, Yigang

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Current Microbiology. Springer. 2021, 78(8), pp. 3192-3200. ISSN 0343-8651. eISSN 1432-0991. Available under: doi: 10.1007/s00284-021-02556-z

Zusammenfassung

Xanthomonas oryzae pv. oryzae (X. oryzae) is a bacterial pathovar of rice diseases all over the world. Owing to emerging antibacterial resistance, phage therapies have gained significant attention to treat various bacterial infections. Nevertheless, comprehensive research is needed for their use as a safe biocontrol agent. In this study, isolation and characterization of a novel phage Xoo-sp15, that infects X. oryzae was ascertained through experimental and bioinformatics analyses to determine its virulent potency and reliability. High throughput sequencing demonstrated that Xoo-sp15 has a dsDNA genome with a total size of 157,091 bp and 39.9% GC content lower than its host (63.6%). Morphological and phylogenetic analyses characterized it as a new member of the Bastille-like group within the family Herelleviridae. In silico analysis revealed that it contains 229 open reading frames and 16 tRNAs. Additionally, this novel phage does not contain any resistant determinants and can infect nine X. oryzae strains. Therefore, Xoo-sp15 has the potential to serve as a novel candidate for phage therapy.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

NAZIR, Amina, Zhaoxia DONG, Jin LIU, Rana Adnan TAHIR, Neelma ASHRAF, Hong QING, Donghai PENG, Yigang TONG, 2021. Isolation, Characterization, and Genome Sequence Analysis of a Novel Lytic Phage, Xoo-sp15 Infecting Xanthomonas oryzae pv. oryzae. In: Current Microbiology. Springer. 2021, 78(8), pp. 3192-3200. ISSN 0343-8651. eISSN 1432-0991. Available under: doi: 10.1007/s00284-021-02556-z

BibTex

@article{Nazir2021-08Isola-58228,
  year={2021},
  doi={10.1007/s00284-021-02556-z},
  title={Isolation, Characterization, and Genome Sequence Analysis of a Novel Lytic Phage, Xoo-sp15 Infecting Xanthomonas oryzae pv. oryzae},
  number={8},
  volume={78},
  issn={0343-8651},
  journal={Current Microbiology},
  pages={3192--3200},
  author={Nazir, Amina and Dong, Zhaoxia and Liu, Jin and Tahir, Rana Adnan and Ashraf, Neelma and Qing, Hong and Peng, Donghai and Tong, Yigang}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/58228">
    <dc:language>eng</dc:language>
    <dc:contributor>Tong, Yigang</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-08-03T14:41:38Z</dc:date>
    <dc:creator>Qing, Hong</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Ashraf, Neelma</dc:contributor>
    <dc:creator>Nazir, Amina</dc:creator>
    <dcterms:abstract xml:lang="eng">Xanthomonas oryzae pv. oryzae (X. oryzae) is a bacterial pathovar of rice diseases all over the world. Owing to emerging antibacterial resistance, phage therapies have gained significant attention to treat various bacterial infections. Nevertheless, comprehensive research is needed for their use as a safe biocontrol agent. In this study, isolation and characterization of a novel phage Xoo-sp15, that infects X. oryzae was ascertained through experimental and bioinformatics analyses to determine its virulent potency and reliability. High throughput sequencing demonstrated that Xoo-sp15 has a dsDNA genome with a total size of 157,091 bp and 39.9% GC content lower than its host (63.6%). Morphological and phylogenetic analyses characterized it as a new member of the Bastille-like group within the family Herelleviridae. In silico analysis revealed that it contains 229 open reading frames and 16 tRNAs. Additionally, this novel phage does not contain any resistant determinants and can infect nine X. oryzae strains. Therefore, Xoo-sp15 has the potential to serve as a novel candidate for phage therapy.</dcterms:abstract>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Tahir, Rana Adnan</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/58228"/>
    <dc:creator>Peng, Donghai</dc:creator>
    <dc:creator>Ashraf, Neelma</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/58228/3/Nazir_2-kpx7stcu63zl0.pdf"/>
    <dc:contributor>Dong, Zhaoxia</dc:contributor>
    <dc:creator>Liu, Jin</dc:creator>
    <dc:contributor>Qing, Hong</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Liu, Jin</dc:contributor>
    <dc:contributor>Tahir, Rana Adnan</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-08-03T14:41:38Z</dcterms:available>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:title>Isolation, Characterization, and Genome Sequence Analysis of a Novel Lytic Phage, Xoo-sp15 Infecting Xanthomonas oryzae pv. oryzae</dcterms:title>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:issued>2021-08</dcterms:issued>
    <dc:contributor>Nazir, Amina</dc:contributor>
    <dc:creator>Tong, Yigang</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/58228/3/Nazir_2-kpx7stcu63zl0.pdf"/>
    <dc:creator>Dong, Zhaoxia</dc:creator>
    <dc:contributor>Peng, Donghai</dc:contributor>
  </rdf:Description>
</rdf:RDF>

Begutachtet

Ja