Soluble TNF-α but not transmembrane TNF-α sensitizes T cells for enhanced activation-induced cell death
Soluble TNF-α but not transmembrane TNF-α sensitizes T cells for enhanced activation-induced cell death
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Date
2009
Authors
Müller, Stefan
Rihs, Silvia
Dayer Schneider, Johanna M.
Paredes, Bruno E.
Seibold, Ingeborg
Mueller, Christoph
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European Journal of Immunology ; 39 (2009), 11. - pp. 3171-3180. - ISSN 0014-2980. - eISSN 1521-4141
Abstract
In addition to its proinflammatory effects, TNF-α exhibits immunosuppression. Here, we compared the capacities of transmembrane TNF-α (tmTNF) and soluble TNF-α (sTNF) in regulating expansion of activated T cells by apoptosis. Splenic CD4+ T cells from wtTNF, TNF-α-deficient (TNF−/−) and TNF−/− mice expressing a non-cleavable mutant tmTNF showed comparable proliferation rates upon TCR-mediated stimulation. Activation-induced cell death (AICD), however, was significantly attenuated in tmTNF and TNF−/−, compared with wtTNF CD4+ T cells. Addition of sTNF during initial priming was sufficient to enhance susceptibility to AICD in tmTNF and TNF−/− CD4+ T cells to levels seen in wtTNF CD4+ T cells, whereas addition of sTNF only during restimulation failed to enhance AICD. sTNF-induced, enhanced susceptibility to AICD was dependent on both TNF receptors. The reduced susceptibility of tmTNF CD4+ T cells for AICD was also evident in an in vivo model of adoptively transferred CD4+ T-cell-mediated colonic inflammation. Hence, the presence of sTNF during T-cell priming may represent an important mechanism to sensitize activated T cells for apoptosis, thereby attenuating the extent and duration of T-cell reactivities and subsequent T-cell-mediated, excessive inflammation.
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570 Biosciences, Biology
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Activation-induced cell death,CD4+ T cells,Colitis,TNF-a,Transmembrane TNF
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MÜLLER, Stefan, Silvia RIHS, Johanna M. DAYER SCHNEIDER, Bruno E. PAREDES, Ingeborg SEIBOLD, Thomas BRUNNER, Christoph MUELLER, 2009. Soluble TNF-α but not transmembrane TNF-α sensitizes T cells for enhanced activation-induced cell death. In: European Journal of Immunology. 39(11), pp. 3171-3180. ISSN 0014-2980. eISSN 1521-4141. Available under: doi: 10.1002/eji.200939554BibTex
@article{Muller2009-11Solub-14256, year={2009}, doi={10.1002/eji.200939554}, title={Soluble TNF-α but not transmembrane TNF-α sensitizes T cells for enhanced activation-induced cell death}, number={11}, volume={39}, issn={0014-2980}, journal={European Journal of Immunology}, pages={3171--3180}, author={Müller, Stefan and Rihs, Silvia and Dayer Schneider, Johanna M. and Paredes, Bruno E. and Seibold, Ingeborg and Brunner, Thomas and Mueller, Christoph} }
RDF
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