Soluble TNF-α but not transmembrane TNF-α sensitizes T cells for enhanced activation-induced cell death

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2009
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Müller, Stefan
Rihs, Silvia
Dayer Schneider, Johanna M.
Paredes, Bruno E.
Seibold, Ingeborg
Mueller, Christoph
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In addition to its proinflammatory effects, TNF-α exhibits immunosuppression. Here, we compared the capacities of transmembrane TNF-α (tmTNF) and soluble TNF-α (sTNF) in regulating expansion of activated T cells by apoptosis. Splenic CD4+ T cells from wtTNF, TNF-α-deficient (TNF−/−) and TNF−/− mice expressing a non-cleavable mutant tmTNF showed comparable proliferation rates upon TCR-mediated stimulation. Activation-induced cell death (AICD), however, was significantly attenuated in tmTNF and TNF−/−, compared with wtTNF CD4+ T cells. Addition of sTNF during initial priming was sufficient to enhance susceptibility to AICD in tmTNF and TNF−/− CD4+ T cells to levels seen in wtTNF CD4+ T cells, whereas addition of sTNF only during restimulation failed to enhance AICD. sTNF-induced, enhanced susceptibility to AICD was dependent on both TNF receptors. The reduced susceptibility of tmTNF CD4+ T cells for AICD was also evident in an in vivo model of adoptively transferred CD4+ T-cell-mediated colonic inflammation. Hence, the presence of sTNF during T-cell priming may represent an important mechanism to sensitize activated T cells for apoptosis, thereby attenuating the extent and duration of T-cell reactivities and subsequent T-cell-mediated, excessive inflammation.

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570 Biowissenschaften, Biologie
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Activation-induced cell death, CD4+ T cells, Colitis, TNF-a, Transmembrane TNF
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ISO 690MÜLLER, Stefan, Silvia RIHS, Johanna M. DAYER SCHNEIDER, Bruno E. PAREDES, Ingeborg SEIBOLD, Thomas BRUNNER, Christoph MUELLER, 2009. Soluble TNF-α but not transmembrane TNF-α sensitizes T cells for enhanced activation-induced cell death. In: European Journal of Immunology. 2009, 39(11), pp. 3171-3180. ISSN 0014-2980. eISSN 1521-4141. Available under: doi: 10.1002/eji.200939554
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@article{Muller2009-11Solub-14256,
  year={2009},
  doi={10.1002/eji.200939554},
  title={Soluble TNF-α but not transmembrane TNF-α sensitizes T cells for enhanced activation-induced cell death},
  number={11},
  volume={39},
  issn={0014-2980},
  journal={European Journal of Immunology},
  pages={3171--3180},
  author={Müller, Stefan and Rihs, Silvia and Dayer Schneider, Johanna M. and Paredes, Bruno E. and Seibold, Ingeborg and Brunner, Thomas and Mueller, Christoph}
}
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