Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase

Leist, Marcel; Single, Barbara; Künstle, Gerald; Volbracht, Christiane; Hentze, Hannes; Nicotera, Pierluigi

Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

1997

Autor:innen

Leist, Marcel

Single, Barbara

Künstle, Gerald

Volbracht, Christiane

Hentze, Hannes

Nicotera, Pierluigi

DOI (zitierfähiger Link)

https://doi.org/10.1006/bbrc.1997.6491

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Biochemical and Biophysical Research Communications. Elsevier. 1997, 233(2), pp. 518-522. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1006/bbrc.1997.6491

Zusammenfassung

Cleavage of poly-(ADP-ribose) polymerase is a process occurring early during the execution phase of apoptosis. Although in many experimental systems PARP cleavage indicates a point of no return, the significance of this proteolytic step for apoptosis remains unclear. Here we compare the susceptibility of cells from wild-type mice and PARP−/− mice to several inducers of apoptosis. Neither the susceptibility of hepatocytes towards CD95 or TNF-mediated apoptosis nor the activation of PARP-cleaving caspases was modified in PARP−/− liver cells. Thymocytes with either genotype exhibited similar sensitivity to treatments with ceramide, dexamethasone, or etoposide. The sensitivity of primary neurons towards apoptosis induced by staurosporine, colchicine, potassium withdrawal, peroxynitrite, or the neurotoxin MPP⁺ was also unaltered. These data suggest that neither activation nor cleavage of PARP has a causal role in apoptotic cell death of primary, non-transformed cells.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

LEIST, Marcel, Barbara SINGLE, Gerald KÜNSTLE, Christiane VOLBRACHT, Hannes HENTZE, Pierluigi NICOTERA, 1997. Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase. In: Biochemical and Biophysical Research Communications. Elsevier. 1997, 233(2), pp. 518-522. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1006/bbrc.1997.6491

BibTex

@article{Leist1997-04-17Apopt-51280,
  year={1997},
  doi={10.1006/bbrc.1997.6491},
  title={Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase},
  number={2},
  volume={233},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={518--522},
  author={Leist, Marcel and Single, Barbara and Künstle, Gerald and Volbracht, Christiane and Hentze, Hannes and Nicotera, Pierluigi}
}

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