Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase

Lade...
Vorschaubild
Dateien
Zu diesem Dokument gibt es keine Dateien.
Datum
1997
Autor:innen
Single, Barbara
Künstle, Gerald
Volbracht, Christiane
Hentze, Hannes
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Zusammenfassung

Cleavage of poly-(ADP-ribose) polymerase is a process occurring early during the execution phase of apoptosis. Although in many experimental systems PARP cleavage indicates a point of no return, the significance of this proteolytic step for apoptosis remains unclear. Here we compare the susceptibility of cells from wild-type mice and PARP−/− mice to several inducers of apoptosis. Neither the susceptibility of hepatocytes towards CD95 or TNF-mediated apoptosis nor the activation of PARP-cleaving caspases was modified in PARP−/− liver cells. Thymocytes with either genotype exhibited similar sensitivity to treatments with ceramide, dexamethasone, or etoposide. The sensitivity of primary neurons towards apoptosis induced by staurosporine, colchicine, potassium withdrawal, peroxynitrite, or the neurotoxin MPP+ was also unaltered. These data suggest that neither activation nor cleavage of PARP has a causal role in apoptotic cell death of primary, non-transformed cells.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690LEIST, Marcel, Barbara SINGLE, Gerald KÜNSTLE, Christiane VOLBRACHT, Hannes HENTZE, Pierluigi NICOTERA, 1997. Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase. In: Biochemical and Biophysical Research Communications. Elsevier. 1997, 233(2), pp. 518-522. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1006/bbrc.1997.6491
BibTex
@article{Leist1997-04-17Apopt-51280,
  year={1997},
  doi={10.1006/bbrc.1997.6491},
  title={Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase},
  number={2},
  volume={233},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={518--522},
  author={Leist, Marcel and Single, Barbara and Künstle, Gerald and Volbracht, Christiane and Hentze, Hannes and Nicotera, Pierluigi}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/51280">
    <dc:creator>Nicotera, Pierluigi</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-10-09T08:18:04Z</dcterms:available>
    <dc:contributor>Single, Barbara</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/51280"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Nicotera, Pierluigi</dc:contributor>
    <dcterms:title>Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase</dcterms:title>
    <dc:creator>Single, Barbara</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:creator>Leist, Marcel</dc:creator>
    <dc:contributor>Volbracht, Christiane</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-10-09T08:18:04Z</dc:date>
    <dc:creator>Hentze, Hannes</dc:creator>
    <dcterms:abstract xml:lang="eng">Cleavage of poly-(ADP-ribose) polymerase is a process occurring early during the execution phase of apoptosis. Although in many experimental systems PARP cleavage indicates a point of no return, the significance of this proteolytic step for apoptosis remains unclear. Here we compare the susceptibility of cells from wild-type mice and PARP−/− mice to several inducers of apoptosis. Neither the susceptibility of hepatocytes towards CD95 or TNF-mediated apoptosis nor the activation of PARP-cleaving caspases was modified in PARP−/− liver cells. Thymocytes with either genotype exhibited similar sensitivity to treatments with ceramide, dexamethasone, or etoposide. The sensitivity of primary neurons towards apoptosis induced by staurosporine, colchicine, potassium withdrawal, peroxynitrite, or the neurotoxin MPP&lt;sup&gt;+&lt;/sup&gt; was also unaltered. These data suggest that neither activation nor cleavage of PARP has a causal role in apoptotic cell death of primary, non-transformed cells.</dcterms:abstract>
    <dc:contributor>Leist, Marcel</dc:contributor>
    <dc:creator>Volbracht, Christiane</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Hentze, Hannes</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:issued>1997-04-17</dcterms:issued>
    <dc:contributor>Künstle, Gerald</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Künstle, Gerald</dc:creator>
    <dc:language>eng</dc:language>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Ja
Diese Publikation teilen