Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase
Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase
No Thumbnail Available
Files
There are no files associated with this item.
Date
1997
Authors
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
DOI (citable link)
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published
Published in
Biochemical and Biophysical Research Communications ; 233 (1997), 2. - pp. 518-522. - Elsevier. - ISSN 0006-291X. - eISSN 1090-2104
Abstract
Cleavage of poly-(ADP-ribose) polymerase is a process occurring early during the execution phase of apoptosis. Although in many experimental systems PARP cleavage indicates a point of no return, the significance of this proteolytic step for apoptosis remains unclear. Here we compare the susceptibility of cells from wild-type mice and PARP−/− mice to several inducers of apoptosis. Neither the susceptibility of hepatocytes towards CD95 or TNF-mediated apoptosis nor the activation of PARP-cleaving caspases was modified in PARP−/− liver cells. Thymocytes with either genotype exhibited similar sensitivity to treatments with ceramide, dexamethasone, or etoposide. The sensitivity of primary neurons towards apoptosis induced by staurosporine, colchicine, potassium withdrawal, peroxynitrite, or the neurotoxin MPP+ was also unaltered. These data suggest that neither activation nor cleavage of PARP has a causal role in apoptotic cell death of primary, non-transformed cells.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
LEIST, Marcel, Barbara SINGLE, Gerald KÜNSTLE, Christiane VOLBRACHT, Hannes HENTZE, Pierluigi NICOTERA, 1997. Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase. In: Biochemical and Biophysical Research Communications. Elsevier. 233(2), pp. 518-522. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1006/bbrc.1997.6491BibTex
@article{Leist1997-04-17Apopt-51280, year={1997}, doi={10.1006/bbrc.1997.6491}, title={Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase}, number={2}, volume={233}, issn={0006-291X}, journal={Biochemical and Biophysical Research Communications}, pages={518--522}, author={Leist, Marcel and Single, Barbara and Künstle, Gerald and Volbracht, Christiane and Hentze, Hannes and Nicotera, Pierluigi} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/51280"> <dc:creator>Nicotera, Pierluigi</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-10-09T08:18:04Z</dcterms:available> <dc:contributor>Single, Barbara</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/51280"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Nicotera, Pierluigi</dc:contributor> <dcterms:title>Apoptosis in the Absence of Poly- (ADP-ribose) Polymerase</dcterms:title> <dc:creator>Single, Barbara</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:creator>Leist, Marcel</dc:creator> <dc:contributor>Volbracht, Christiane</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-10-09T08:18:04Z</dc:date> <dc:creator>Hentze, Hannes</dc:creator> <dcterms:abstract xml:lang="eng">Cleavage of poly-(ADP-ribose) polymerase is a process occurring early during the execution phase of apoptosis. Although in many experimental systems PARP cleavage indicates a point of no return, the significance of this proteolytic step for apoptosis remains unclear. Here we compare the susceptibility of cells from wild-type mice and PARP−/− mice to several inducers of apoptosis. Neither the susceptibility of hepatocytes towards CD95 or TNF-mediated apoptosis nor the activation of PARP-cleaving caspases was modified in PARP−/− liver cells. Thymocytes with either genotype exhibited similar sensitivity to treatments with ceramide, dexamethasone, or etoposide. The sensitivity of primary neurons towards apoptosis induced by staurosporine, colchicine, potassium withdrawal, peroxynitrite, or the neurotoxin MPP<sup>+</sup> was also unaltered. These data suggest that neither activation nor cleavage of PARP has a causal role in apoptotic cell death of primary, non-transformed cells.</dcterms:abstract> <dc:contributor>Leist, Marcel</dc:contributor> <dc:creator>Volbracht, Christiane</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:contributor>Hentze, Hannes</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dcterms:issued>1997-04-17</dcterms:issued> <dc:contributor>Künstle, Gerald</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:creator>Künstle, Gerald</dc:creator> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
No
Refereed
Yes