Publikation:

AUREOCHROME1a-Mediated Induction of the Diatom-Specific Cyclin dsCYC2 Controls the Onset of Cell Division in Diatoms (Phaeodactylum tricornutum)

Lade...
Vorschaubild

Dateien

Huysman_219607.pdf
Huysman_219607.pdfGröße: 1.08 MBDownloads: 464

Datum

2013

Autor:innen

Huysman, Marie
Fortunato, Antonio
Matthijs, Michiel
Schellenberger Costa, Benjamin
Vanderhaeghen, Rudy
Van den Daele, Hilde
Inzé, Dirk
Bowler, Chris

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

TP 8 Biological function of aureochromes in the diatom Phaeodactylum tricornutum
Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

The Plant Cell. 2013, 25(1), pp. 215-228. ISSN 1040-4651. eISSN 1532-298X. Available under: doi: 10.1105/tpc.112.106377

Zusammenfassung

Cell division in photosynthetic organisms is tightly regulated by light. Although the light dependency of the onset of the cell cycle has been well characterized in various phototrophs, little is known about the cellular signaling cascades connecting light perception to cell cycle activation and progression. Here, we demonstrate that diatom-specific cyclin 2 (dsCYC2) in Phaeodactylum tricornutum displays a transcriptional peak within 15 min after light exposure, long before the onset of cell division. The product of dsCYC2 binds to the cyclin-dependent kinase CDKA1 and can complement G1 cyclin-deficient yeast. Consistent with the role of dsCYC2 in controlling a G1-to-S light-dependent cell cycle checkpoint, dsCYC2 silencing decreases the rate of cell division in diatoms exposed to light-dark cycles but not to constant light. Transcriptional induction of dsCYC2 is triggered by blue light in a fluence rate-dependent manner. Consistent with this, dsCYC2 is a transcriptional target of the blue light sensor AUREOCHROME1a, which functions synergistically with the basic leucine zipper (bZIP) transcription factor bZIP10 to induce dsCYC2 transcription. The functional characterization of a cyclin whose transcription is controlled by light and whose activity connects light signaling to cell cycle progression contributes significantly to our understanding of the molecular mechanisms underlying light-dependent cell cycle onset in diatoms.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690HUYSMAN, Marie, Antonio FORTUNATO, Michiel MATTHIJS, Benjamin SCHELLENBERGER COSTA, Rudy VANDERHAEGHEN, Hilde VAN DEN DAELE, Matthias SACHSE, Dirk INZÉ, Chris BOWLER, Peter G. KROTH, Christian WILHELM, Angela FALCIATORE, Wim VYVERMAN, Lieven DE VEYLDER, 2013. AUREOCHROME1a-Mediated Induction of the Diatom-Specific Cyclin dsCYC2 Controls the Onset of Cell Division in Diatoms (Phaeodactylum tricornutum). In: The Plant Cell. 2013, 25(1), pp. 215-228. ISSN 1040-4651. eISSN 1532-298X. Available under: doi: 10.1105/tpc.112.106377
BibTex
@article{Huysman2013-01AUREO-21960,
  year={2013},
  doi={10.1105/tpc.112.106377},
  title={AUREOCHROME1a-Mediated Induction of the Diatom-Specific Cyclin dsCYC2 Controls the Onset of Cell Division in Diatoms (Phaeodactylum tricornutum)},
  number={1},
  volume={25},
  issn={1040-4651},
  journal={The Plant Cell},
  pages={215--228},
  author={Huysman, Marie and Fortunato, Antonio and Matthijs, Michiel and Schellenberger Costa, Benjamin and Vanderhaeghen, Rudy and Van den Daele, Hilde and Sachse, Matthias and Inzé, Dirk and Bowler, Chris and Kroth, Peter G. and Wilhelm, Christian and Falciatore, Angela and Vyverman, Wim and De Veylder, Lieven}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/21960">
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-03-13T15:20:10Z</dcterms:available>
    <dc:creator>Van den Daele, Hilde</dc:creator>
    <dc:contributor>Vanderhaeghen, Rudy</dc:contributor>
    <dc:creator>Matthijs, Michiel</dc:creator>
    <dc:creator>Schellenberger Costa, Benjamin</dc:creator>
    <dc:contributor>Kroth, Peter G.</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-03-13T15:20:10Z</dc:date>
    <dc:creator>Vanderhaeghen, Rudy</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Sachse, Matthias</dc:creator>
    <dc:creator>Falciatore, Angela</dc:creator>
    <dc:creator>Fortunato, Antonio</dc:creator>
    <dc:contributor>Matthijs, Michiel</dc:contributor>
    <dcterms:issued>2013-01</dcterms:issued>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21960/2/Huysman_219607.pdf"/>
    <dc:contributor>De Veylder, Lieven</dc:contributor>
    <dc:contributor>Wilhelm, Christian</dc:contributor>
    <dc:contributor>Schellenberger Costa, Benjamin</dc:contributor>
    <dc:creator>Inzé, Dirk</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Kroth, Peter G.</dc:creator>
    <dc:contributor>Sachse, Matthias</dc:contributor>
    <dc:creator>Bowler, Chris</dc:creator>
    <dc:contributor>Van den Daele, Hilde</dc:contributor>
    <dc:creator>Huysman, Marie</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>De Veylder, Lieven</dc:creator>
    <dc:contributor>Fortunato, Antonio</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/21960"/>
    <dcterms:bibliographicCitation>The Plant Cell ; 25 (2013), 1. - S. 215-228</dcterms:bibliographicCitation>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Inzé, Dirk</dc:contributor>
    <dcterms:abstract xml:lang="eng">Cell division in photosynthetic organisms is tightly regulated by light. Although the light dependency of the onset of the cell cycle has been well characterized in various phototrophs, little is known about the cellular signaling cascades connecting light perception to cell cycle activation and progression. Here, we demonstrate that diatom-specific cyclin 2 (dsCYC2) in Phaeodactylum tricornutum displays a transcriptional peak within 15 min after light exposure, long before the onset of cell division. The product of dsCYC2 binds to the cyclin-dependent kinase CDKA1 and can complement G1 cyclin-deficient yeast. Consistent with the role of dsCYC2 in controlling a G1-to-S light-dependent cell cycle checkpoint, dsCYC2 silencing decreases the rate of cell division in diatoms exposed to light-dark cycles but not to constant light. Transcriptional induction of dsCYC2 is triggered by blue light in a fluence rate-dependent manner. Consistent with this, dsCYC2 is a transcriptional target of the blue light sensor AUREOCHROME1a, which functions synergistically with the basic leucine zipper (bZIP) transcription factor bZIP10 to induce dsCYC2 transcription. The functional characterization of a cyclin whose transcription is controlled by light and whose activity connects light signaling to cell cycle progression contributes significantly to our understanding of the molecular mechanisms underlying light-dependent cell cycle onset in diatoms.</dcterms:abstract>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Huysman, Marie</dc:contributor>
    <dc:language>eng</dc:language>
    <dc:creator>Vyverman, Wim</dc:creator>
    <dcterms:title>AUREOCHROME1a-Mediated Induction of the Diatom-Specific Cyclin dsCYC2 Controls the Onset of Cell Division in Diatoms (Phaeodactylum tricornutum)</dcterms:title>
    <dc:creator>Wilhelm, Christian</dc:creator>
    <dc:contributor>Vyverman, Wim</dc:contributor>
    <dc:contributor>Falciatore, Angela</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21960/2/Huysman_219607.pdf"/>
    <dc:contributor>Bowler, Chris</dc:contributor>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen