Immunocytochemical localization of pectinesterases in hyphae of Phytophthora infestans

Förster, Helga; Mendgen, Kurt

Immunocytochemical localization of pectinesterases in hyphae of Phytophthora infestans

Files

Immunocytochemical_1987.pdf(635.12 KB)

Date

1987

Authors

Förster, Helga

Mendgen, Kurt

Publication type

Journal article

Published in

Canadian Journal of Botany ; 65 (1987), 12. - pp. 2607-2613. - ISSN 0008-4026. - eISSN 1480-3305

Abstract

Evidence for the localization of extracellular pectinesterases was obtained in hyphae of Phytophthora infestans with the antibody - protein A - gold technique. Hyphae were fixed in 1 % formaldehyde and 0.5 % glutaraldehyde, and the immunocytochemical localization was done on ultrathin sections of tissue embedded at low temperature in Lowicryl K4M. Gold particles were mainly present over three different types of vesicles and over dictyosomes. In older parts of the hyphae, the plasma membrane was heavily labeled. This might suggest that the hyphal cell wall in subapical regions is not as permeable as in the hyphal tips.

Summary in another language

L'on a obtenu une preuve de la localisation de pectine-estérases extracellulaires dans les hyphes de Phyllohthora infestans à l'aide de la technique anticorps - protéine A - or. Les hyphes sont fixés dans la formaldéhyde 1 % et la glutaraldéhyde 0,5 %. La localisation immunocytochimique est réalisée sur des sections ultraminces de tissus enrobés à basse température dans du Lowicryl K4M. Les particules d'or sont surtout présentes sur trois types différents de vésicules et sur les dictyosomes. Dans les parties plus agées des hyphes, la membrane plasmique est fortement marquée. Ceci pourrait suggérer que la paroi cellulaire de l'hyphe dans les régions sous-apicales est moins perméable qu'aux apex des hyphes.

Subject (DDC)

570 Biosciences, Biology

Cite This

ISO 690

FÖRSTER, Helga, Kurt MENDGEN, 1987. Immunocytochemical localization of pectinesterases in hyphae of Phytophthora infestans. In: Canadian Journal of Botany. 65(12), pp. 2607-2613. ISSN 0008-4026. eISSN 1480-3305. Available under: doi: 10.1139/b87-351

BibTex

@article{Forster1987Immun-6800,
  year={1987},
  doi={10.1139/b87-351},
  title={Immunocytochemical localization of pectinesterases in hyphae of Phytophthora infestans},
  number={12},
  volume={65},
  issn={0008-4026},
  journal={Canadian Journal of Botany},
  pages={2607--2613},
  author={Förster, Helga and Mendgen, Kurt}
}

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