@article{Reuter2013-04Ident-22936,
  year={2013},
  doi={10.1128/EC.00324-12},
  title={Identification, Localization, and Functional Implications of the Microdomain-Forming Stomatin Family in the Ciliated Protozoan Paramecium tetraurelia},
  number={4},
  volume={12},
  issn={1535-9778},
  journal={Eukaryotic Cell},
  pages={529--544},
  author={Reuter, Alexander and Stürmer, Claudia and Plattner, Helmut}
}

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    <dc:contributor>Reuter, Alexander</dc:contributor>
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    <dcterms:abstract xml:lang="eng">The SPFH protein superfamily is assumed to occur universally in eukaryotes, but information from protozoa is scarce. In the Paramecium genome, we found only Stomatins, 20 paralogs grouped in 8 families, STO1 to STO8. According to cDNA analysis, all are expressed, and molecular modeling shows the typical SPFH domain structure for all subgroups. For further analysis we used family-specific sequences for fluorescence and immunogold labeling, gene silencing, and functional tests. With all family members tested, we found a patchy localization at/near the cell surface and on vesicles. The StO1 and StO4 families are also associated with the contractile vacuole complex. StO4 also makes puncta on some food vacuoles and is abundant on vesicles recycling from the release site of spent food vacuoles to the site of nascent food vacuole formation. Silencing of the STO1 family reduces mechanosensitivity (ciliary reversal upon touching an obstacle), thus suggesting relevance for positioning of mechanosensitive channels in the plasmalemma. Silencing of STO4 members increases pulsation frequency of the contractile vacuole complex and reduces phagocytotic activity of Paramecium cells. In summary, StO1 and StO4 members seem to be involved in positioning specific superficial and intracellular microdomain-based membrane components whose functions may depend on mechanosensation (extracellular stimuli and internal osmotic pressure).</dcterms:abstract>
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    <dc:creator>Reuter, Alexander</dc:creator>
    <dc:creator>Stürmer, Claudia</dc:creator>
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    <dc:contributor>Stürmer, Claudia</dc:contributor>
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    <dc:contributor>Plattner, Helmut</dc:contributor>
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    <dc:creator>Plattner, Helmut</dc:creator>
    <dcterms:bibliographicCitation>Eukaryotic Cell ; 12 (2013), 4. - S. 529-544</dcterms:bibliographicCitation>
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    <dcterms:title>Identification, Localization, and Functional Implications of the Microdomain-Forming Stomatin Family in the Ciliated Protozoan Paramecium tetraurelia</dcterms:title>
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