## Deciphering Epigenetic Cytosine Modifications by Direct Molecular Recognition

No Thumbnail Available
##### Files
There are no files associated with this item.
2015
Journal article
##### Published in
ACS Chemical Biology ; 10 (2015), 7. - pp. 1580-1589. - ISSN 1554-8929. - eISSN 1554-8937
##### Abstract
Epigenetic modification at the 5-position of cytosine is a key regulatory element of mammalian gene expression with important roles in genome stability, development, and disease. The repertoire of cytosine modifications has long been confined to only 5-methylcytosine (mC) but has recently been expanded by the discovery of 5-hydroxymethyl-, 5-formyl-, and 5-carboxylcytosine. These are key intermediates of active mC demethylation but may additionally represent new epigenetic marks with distinct biological roles. This leap in chemical complexity of epigenetic cytosine modifications has not only created a pressing need for analytical approaches that enable unraveling of their functions, it has also created new challenges for such analyses with respect to sensitivity and selectivity. The crucial step of any such approach that defines its analytic potential is the strategy used for the actual differentiation of the cytosine 5-modifications from one another, and this selectivity can in principle be provided either by chemoselective conversions or by selective, molecular recognition events. While the former strategy has been particularly successful for accurate genomic profiling of cytosine modifications in vitro, the latter strategy provides interesting perspectives for simplified profiling of natural, untreated DNA, as well as for emerging applications such as single cell analysis and the monitoring of cytosine modification in vivo. We here review analytical techniques for the deciphering of epigenetic cytosine modifications with an emphasis on approaches that are based on the direct molecular recognition of these modifications in DNA.
540 Chemistry
##### Cite This
ISO 690KUBIK, Grzegorz, Daniel SUMMERER, 2015. Deciphering Epigenetic Cytosine Modifications by Direct Molecular Recognition. In: ACS Chemical Biology. 10(7), pp. 1580-1589. ISSN 1554-8929. eISSN 1554-8937. Available under: doi: 10.1021/acschembio.5b00158
BibTex
@article{Kubik2015-05-01Decip-30993,
year={2015},
doi={10.1021/acschembio.5b00158},
title={Deciphering Epigenetic Cytosine Modifications by Direct Molecular Recognition},
number={7},
volume={10},
issn={1554-8929},
journal={ACS Chemical Biology},
pages={1580--1589},
author={Kubik, Grzegorz and Summerer, Daniel}
}

RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<dc:language>eng</dc:language>
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-05-21T06:34:33Z</dcterms:available>
<dc:contributor>Kubik, Grzegorz</dc:contributor>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
<dcterms:abstract xml:lang="eng">Epigenetic modification at the 5-position of cytosine is a key regulatory element of mammalian gene expression with important roles in genome stability, development, and disease. The repertoire of cytosine modifications has long been confined to only 5-methylcytosine (mC) but has recently been expanded by the discovery of 5-hydroxymethyl-, 5-formyl-, and 5-carboxylcytosine. These are key intermediates of active mC demethylation but may additionally represent new epigenetic marks with distinct biological roles. This leap in chemical complexity of epigenetic cytosine modifications has not only created a pressing need for analytical approaches that enable unraveling of their functions, it has also created new challenges for such analyses with respect to sensitivity and selectivity. The crucial step of any such approach that defines its analytic potential is the strategy used for the actual differentiation of the cytosine 5-modifications from one another, and this selectivity can in principle be provided either by chemoselective conversions or by selective, molecular recognition events. While the former strategy has been particularly successful for accurate genomic profiling of cytosine modifications in vitro, the latter strategy provides interesting perspectives for simplified profiling of natural, untreated DNA, as well as for emerging applications such as single cell analysis and the monitoring of cytosine modification in vivo. We here review analytical techniques for the deciphering of epigenetic cytosine modifications with an emphasis on approaches that are based on the direct molecular recognition of these modifications in DNA.</dcterms:abstract>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
<bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/30993"/>
<dc:creator>Summerer, Daniel</dc:creator>
<dcterms:issued>2015-05-01</dcterms:issued>
<dc:creator>Kubik, Grzegorz</dc:creator>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
<dc:contributor>Summerer, Daniel</dc:contributor>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-05-21T06:34:33Z</dc:date>
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
<dcterms:title>Deciphering Epigenetic Cytosine Modifications by Direct Molecular Recognition</dcterms:title>
</rdf:Description>
</rdf:RDF>

Yes