Living on Sulfate : Three-Dimensional Structure and Spectroscopy of Adenosine 5'-Phosphosulfate Reductase and Dissimilatory Sulfite Reductase

Fritz, Günter; Schiffer, Alexander; Behrens, Anke; Büchert, Thomas; Ermler, Ulrich; Kroneck, Peter M. H.

Living on Sulfate : Three-Dimensional Structure and Spectroscopy of Adenosine 5'-Phosphosulfate Reductase and Dissimilatory Sulfite Reductase

Dateien

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Datum

2008

Autor:innen

Fritz, Günter

Schiffer, Alexander

Behrens, Anke

Büchert, Thomas

Ermler, Ulrich

Kroneck, Peter M. H.

DOI (zitierfähiger Link)

https://doi.org/10.1007/978-3-540-72682-1_2

Sammlungen

Biologie

Publikationstyp

Beitrag zu einem Sammelband

Publikationsstatus

Published

Erschienen in

DAHL, Christiane, ed. and others. Microbial Sulfur Metabolism. Berlin: Springer, 2008, pp. 13-23. ISBN 978-3-540-72679-1. Available under: doi: 10.1007/978-3-540-72682-1_2

Zusammenfassung

The reduction of sulfate to sulfide and the reverse reaction are widespread biological processes. Hereby, microorganisms play a central role. Plants also reduce sulfate for the purpose of biosynthesis, and both plants and animals convert reduced sulfur compounds to sulfate. Sulfate respiration is used for energy conservation by strictly anaerobic bacteria and archaea. The redox equivalents generated by the oxidation of organic compounds are transferred to sulfate as the terminal electron acceptor. There are three key enzymes localized in the cytoplasm or at the cytoplasmic aspect of the inner membrane: ATP sulfurylase (ATPS), adenosine 5´-phosphosulfate reductase (APSR), and dissimilatory sulfite reductase (SIR). Sulfate (S6+) cannot be directly reduced by dihydrogen or organic acids, it has to be activated to adenosine 5´-phosphosulfate (APS) catalyzed by ATPS. The enzyme APSR (cofactors flavin adenine dinucleotide, [4Fe4S]) catalyzes the conversion of APS to sulfite (S4+) and AMP, followed by the complex multicomponent enzyme SIR (cofactors siroheme, [4Fe4S]) which catalyzes the reduction of sulfite (S4+) to sulfide (S2?). In this contribution we present the three-dimensional structures of APSR from Archaeoglobus fulgidus and of catalytically relevant reaction intermediates. In addition, we discuss spectroscopic and structural data of SIR purified from this organism.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

FRITZ, Günter, Alexander SCHIFFER, Anke BEHRENS, Thomas BÜCHERT, Ulrich ERMLER, Peter M. H. KRONECK, 2008. Living on Sulfate : Three-Dimensional Structure and Spectroscopy of Adenosine 5'-Phosphosulfate Reductase and Dissimilatory Sulfite Reductase. In: DAHL, Christiane, ed. and others. Microbial Sulfur Metabolism. Berlin: Springer, 2008, pp. 13-23. ISBN 978-3-540-72679-1. Available under: doi: 10.1007/978-3-540-72682-1_2

BibTex

@incollection{Fritz2008Livin-38513,
  year={2008},
  doi={10.1007/978-3-540-72682-1_2},
  title={Living on Sulfate : Three-Dimensional Structure and Spectroscopy of Adenosine 5'-Phosphosulfate Reductase and Dissimilatory Sulfite Reductase},
  isbn={978-3-540-72679-1},
  publisher={Springer},
  address={Berlin},
  booktitle={Microbial Sulfur Metabolism},
  pages={13--23},
  editor={Dahl, Christiane},
  author={Fritz, Günter and Schiffer, Alexander and Behrens, Anke and Büchert, Thomas and Ermler, Ulrich and Kroneck, Peter M. H.}
}

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