Interactions between Flavins and Quadruplex Nucleic Acids

Merkle, Tobias; Sinn, Malte; Hartig, Jörg S.

Interactions between Flavins and Quadruplex Nucleic Acids

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Date

2015

Publication type

Journal article

Publication status

Published

Published in

ChemBioChem ; 16 (2015), 17. - pp. 2437-2440. - ISSN 1439-4227. - eISSN 1439-7633

Abstract

Quadruplex nucleic acids are widespread in genomes. They influence processes such as transcription, translation, replication, recombination, and the regulation of gene expression. Several synthetic ligands have been demonstrated to target quadruplex nucleic acids. However, only very few metabolites have been reported to interact with quadruplexes. In principle, an intracellular metabolite that selectively binds to four-stranded sequences could modulate quadruplex formation, stability, and thus functions in a riboswitch (or deoxyriboswitch) manner. Here we report quadruplex interactions with flavin derivatives such as FMN and FAD. The affinities were highest with parallel quadruplexes, with low (14-20 μM) dissociation constants. Taking into account combined intracellular flavin concentrations of 243 μM in E. coli, the observed interactions in principle open up the possibility of flavin levels affecting gene expression and other processes by modulating quadruplex formation.

Subject (DDC)

540 Chemistry

Keywords

DNA structures, FAD, flavin metabolism, nucleic acids, riboswitch

Cite This

ISO 690

MERKLE, Tobias, Malte SINN, Jörg S. HARTIG, 2015. Interactions between Flavins and Quadruplex Nucleic Acids. In: ChemBioChem. 16(17), pp. 2437-2440. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201500463

BibTex

@article{Merkle2015Inter-32338,
  year={2015},
  doi={10.1002/cbic.201500463},
  title={Interactions between Flavins and Quadruplex Nucleic Acids},
  number={17},
  volume={16},
  issn={1439-4227},
  journal={ChemBioChem},
  pages={2437--2440},
  author={Merkle, Tobias and Sinn, Malte and Hartig, Jörg S.}
}

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Bibliography of Konstanz

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