β-NAD as a building block in natural product biosynthesis
β-NAD as a building block in natural product biosynthesis
Loading...
Date
2021
Authors
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
DOI (citable link)
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published
Published in
Nature ; 600 (2021), 7890. - pp. 754-758. - Springer Nature. - ISSN 0028-0836. - eISSN 1476-4687
Abstract
β-Nicotinamide adenine dinucleotide (β-NAD) is a pivotal metabolite for all living organisms and functions as a diffusible electron acceptor and carrier in the catabolic arms of metabolism1,2. Furthermore, β-NAD is involved in diverse epigenetic, immunological and stress-associated processes, where it is known to be sacrificially utilized as an ADP-ribosyl donor for protein and DNA modifications, or the generation of cell-signalling molecules3,4. Here we report the function of β-NAD in secondary metabolite biosynthetic pathways, in which the nicotinamide dinucleotide framework is heavily decorated and serves as a building block for the assembly of a novel class of natural products. The gatekeeping enzyme of the discovered pathway (SbzP) catalyses a pyridoxal phosphate-dependent [3+2]-annulation reaction between β-NAD and S-adenosylmethionine, generating a 6-azatetrahydroindane scaffold. Members of this novel family of β-NAD-tailoring enzymes are widely distributed in the bacterial kingdom and are encoded in diverse biosynthetic gene clusters. The findings of this work set the stage for the discovery and exploitation of β-NAD-derived natural products.
Summary in another language
Subject (DDC)
540 Chemistry
Keywords
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
BARRA, Lena, Takayoshi AWAKAWA, Kohei SHIRAI, Zhijuan HU, Ghader BASHIRI, Ikuro ABE, 2021. β-NAD as a building block in natural product biosynthesis. In: Nature. Springer Nature. 600(7890), pp. 754-758. ISSN 0028-0836. eISSN 1476-4687. Available under: doi: 10.1038/s41586-021-04214-7BibTex
@article{Barra2021build-57549, year={2021}, doi={10.1038/s41586-021-04214-7}, title={β-NAD as a building block in natural product biosynthesis}, number={7890}, volume={600}, issn={0028-0836}, journal={Nature}, pages={754--758}, author={Barra, Lena and Awakawa, Takayoshi and Shirai, Kohei and Hu, Zhijuan and Bashiri, Ghader and Abe, Ikuro} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/57549"> <dc:contributor>Bashiri, Ghader</dc:contributor> <dc:contributor>Barra, Lena</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/57549"/> <dc:contributor>Shirai, Kohei</dc:contributor> <dc:creator>Shirai, Kohei</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-05-17T08:53:07Z</dcterms:available> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-05-17T08:53:07Z</dc:date> <dc:creator>Abe, Ikuro</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/57549/1/Barra_2-mws0bdkyxovg2.pdf"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/57549/1/Barra_2-mws0bdkyxovg2.pdf"/> <dcterms:title>β-NAD as a building block in natural product biosynthesis</dcterms:title> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:contributor>Hu, Zhijuan</dc:contributor> <dcterms:issued>2021</dcterms:issued> <dc:creator>Barra, Lena</dc:creator> <dc:contributor>Awakawa, Takayoshi</dc:contributor> <dc:creator>Bashiri, Ghader</dc:creator> <dc:language>eng</dc:language> <foaf:homepage rdf:resource="http://localhost:8080/"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Abe, Ikuro</dc:contributor> <dcterms:abstract xml:lang="eng">β-Nicotinamide adenine dinucleotide (β-NAD) is a pivotal metabolite for all living organisms and functions as a diffusible electron acceptor and carrier in the catabolic arms of metabolism1,2. Furthermore, β-NAD is involved in diverse epigenetic, immunological and stress-associated processes, where it is known to be sacrificially utilized as an ADP-ribosyl donor for protein and DNA modifications, or the generation of cell-signalling molecules3,4. Here we report the function of β-NAD in secondary metabolite biosynthetic pathways, in which the nicotinamide dinucleotide framework is heavily decorated and serves as a building block for the assembly of a novel class of natural products. The gatekeeping enzyme of the discovered pathway (SbzP) catalyses a pyridoxal phosphate-dependent [3+2]-annulation reaction between β-NAD and S-adenosylmethionine, generating a 6-azatetrahydroindane scaffold. Members of this novel family of β-NAD-tailoring enzymes are widely distributed in the bacterial kingdom and are encoded in diverse biosynthetic gene clusters. The findings of this work set the stage for the discovery and exploitation of β-NAD-derived natural products.</dcterms:abstract> <dc:creator>Hu, Zhijuan</dc:creator> <dc:creator>Awakawa, Takayoshi</dc:creator> <dc:rights>terms-of-use</dc:rights> </rdf:Description> </rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
No
Refereed
Yes