Analysis of Cell Death Induction in Intestinal Organoids In Vitro

Grabinger, Thomas; Delgado, M. Eugenia; Brunner, Thomas

Analysis of Cell Death Induction in Intestinal Organoids In Vitro

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2016

Publikationstyp

Beitrag zu einem Sammelband

Publikationsstatus

Published

Erschienen in

PUTHALAKATH, Hamsa, ed. and others. Programmed cell death : methods and protocols. New York, NY: Humana Press, 2016, pp. 83-93. Methods in Molecular Biology. 1419. ISBN 978-1-4939-3579-6. Available under: doi: 10.1007/978-1-4939-3581-9_7

Zusammenfassung

The intestinal epithelium has an important function in the absorption of nutrients contained in the food. Furthermore, it also has an important barrier function, preventing luminal pathogens from entering the bloodstream. This single cell layer epithelium is quite sensitive to various cell death-promoting triggers, including drugs, irradiation, and TNF family members, leading to loss of barrier integrity, epithelial erosion, inflammation, malabsorption, and diarrhea. In order to assess the intestinal epithelium-damaging potential of treatments and substances specific test systems are required. As intestinal tumor cell lines are a poor substitute for primary intestinal epithelial cells, and in vivo experiments in mice are costly and often unethical, the use of intestinal organoids cultured from intestinal crypts provide an ideal tool to study cell death induction and mechanisms in primary intestinal epithelial cells. This protocol describes the isolation and culture of intestinal organoids from murine small intestinal crypts, and the quantitative assessment of cell death induction in these organoids.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

GRABINGER, Thomas, M. Eugenia DELGADO, Thomas BRUNNER, 2016. Analysis of Cell Death Induction in Intestinal Organoids In Vitro. In: PUTHALAKATH, Hamsa, ed. and others. Programmed cell death : methods and protocols. New York, NY: Humana Press, 2016, pp. 83-93. Methods in Molecular Biology. 1419. ISBN 978-1-4939-3579-6. Available under: doi: 10.1007/978-1-4939-3581-9_7

BibTex

@incollection{Grabinger2016-04-24Analy-35488,
  year={2016},
  doi={10.1007/978-1-4939-3581-9_7},
  title={Analysis of Cell Death Induction in Intestinal Organoids In Vitro},
  number={1419},
  isbn={978-1-4939-3579-6},
  publisher={Humana Press},
  address={New York, NY},
  series={Methods in Molecular Biology},
  booktitle={Programmed cell death : methods and protocols},
  pages={83--93},
  editor={Puthalakath, Hamsa},
  author={Grabinger, Thomas and Delgado, M. Eugenia and Brunner, Thomas}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/35488">
    <dc:creator>Delgado, M. Eugenia</dc:creator>
    <dc:contributor>Grabinger, Thomas</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:abstract xml:lang="eng">The intestinal epithelium has an important function in the absorption of nutrients contained in the food. Furthermore, it also has an important barrier function, preventing luminal pathogens from entering the bloodstream. This single cell layer epithelium is quite sensitive to various cell death-promoting triggers, including drugs, irradiation, and TNF family members, leading to loss of barrier integrity, epithelial erosion, inflammation, malabsorption, and diarrhea. In order to assess the intestinal epithelium-damaging potential of treatments and substances specific test systems are required. As intestinal tumor cell lines are a poor substitute for primary intestinal epithelial cells, and in vivo experiments in mice are costly and often unethical, the use of intestinal organoids cultured from intestinal crypts provide an ideal tool to study cell death induction and mechanisms in primary intestinal epithelial cells. This protocol describes the isolation and culture of intestinal organoids from murine small intestinal crypts, and the quantitative assessment of cell death induction in these organoids.</dcterms:abstract>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Brunner, Thomas</dc:creator>
    <dcterms:title>Analysis of Cell Death Induction in Intestinal Organoids In Vitro</dcterms:title>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-10-06T08:41:13Z</dcterms:available>
    <dc:creator>Grabinger, Thomas</dc:creator>
    <dc:contributor>Brunner, Thomas</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-10-06T08:41:13Z</dc:date>
    <dc:language>eng</dc:language>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/35488"/>
    <dc:contributor>Delgado, M. Eugenia</dc:contributor>
    <dcterms:issued>2016-04-24</dcterms:issued>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja