Activation of Acetone and Other Simple Ketones in Anaerobic Bacteria

Heider, Johann; Schühle, Karola; Frey, Jasmin; Schink, Bernhard

Activation of Acetone and Other Simple Ketones in Anaerobic Bacteria

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Date

2016

Authors

Heider, Johann

Schühle, Karola

Frey, Jasmin

Schink, Bernhard

DOI (citable link)

10.1159/000441500

Collections

Biologie

Publication type

Journal article

Publication status

Published

Published in

Journal of Molecular Microbiology and Biotechnology ; 26 (2016), 1-3. - pp. 152-164. - ISSN 1464-1801. - eISSN 1660-2412

Abstract

Acetone and other ketones are activated for subsequent degradation through carboxylation by many nitrate-reducing, phototrophic, and obligately aerobic bacteria. Acetone carboxylation leads to acetoacetate, which is subsequently activated to a thioester and degraded via thiolysis. Two different types of acetone carboxylases have been described, which require either 2 or 4 ATP equivalents as an energy supply for the carboxylation reaction. Both enzymes appear to combine acetone enolphosphate with carbonic phosphate to form acetoacetate. A similar but more complex enzyme is known to carboxylate the aromatic ketone acetophenone, a metabolic intermediate in anaerobic ethylbenzene metabolism in denitrifying bacteria, with simultaneous hydrolysis of 2 ATP to 2 ADP. Obligately anaerobic sulfate-reducing bacteria activate acetone to a four-carbon compound as well, but via a different process than bicarbonate- or CO2-dependent carboxylation. The present evidence indicates that either carbon monoxide or a formyl residue is used as a cosubstrate, and that the overall ATP expenditure of this pathway is substantially lower than in the known acetone carboxylase reactions.

Subject (DDC)

570 Biosciences, Biology

Cite This

ISO 690

HEIDER, Johann, Karola SCHÜHLE, Jasmin FREY, Bernhard SCHINK, 2016. Activation of Acetone and Other Simple Ketones in Anaerobic Bacteria. In: Journal of Molecular Microbiology and Biotechnology. 26(1-3), pp. 152-164. ISSN 1464-1801. eISSN 1660-2412. Available under: doi: 10.1159/000441500

BibTex

@article{Heider2016-03-10Activ-33891,
  year={2016},
  doi={10.1159/000441500},
  title={Activation of Acetone and Other Simple Ketones in Anaerobic Bacteria},
  number={1-3},
  volume={26},
  issn={1464-1801},
  journal={Journal of Molecular Microbiology and Biotechnology},
  pages={152--164},
  author={Heider, Johann and Schühle, Karola and Frey, Jasmin and Schink, Bernhard}
}

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