Lipopolysaccharide induces intestinal glucocorticoid synthesis in a TNFalpha-dependent manner
Lipopolysaccharide induces intestinal glucocorticoid synthesis in a TNFalpha-dependent manner
Loading...
Date
2010
Authors
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
DOI (citable link)
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published in
The FASEB Journal ; 24 (2010), 5. - pp. 1340-1346. - ISSN 0892-6638. - eISSN 1530-6860
Abstract
Stringent control of immune responses in the intestinal mucosa is critical for the maintenance of immune homeostasis and prevention of tissue damage, such as observed during inflammatory bowel disease. Intestinal epithelial cells, primarily thought to form a simple physical barrier, critically regulate intestinal immune cell functions by producing immunoregulatory glucocorticoids on T-cell activation. In this study we investigated whether stimulation of cells of the innate immune system results in the induction of intestinal glucocorticoids synthesis and what role TNF-α plays in this process. Stimulation of the innate immune system with lipopolysaccharide (LPS) led to an up-regulation of colonic steroidogenic enzymes and the induction of intestinal glucocorticoid synthesis. The observed induction was dependent on macrophage effector functions, as depletion of macrophages using clodronate-containing liposomes, but not absence of T and B cells, inhibited intestinal glucocorticoid synthesis. LPS-induced glucocorticoid synthesis was critically dependent on TNF-α as it was significantly decreased in TNF-α-deficient animals. Both TNF receptor-1 and -2 were found to be equally involved in LPS- and T-cell-induced intestinal GC synthesis. These results describe a novel and critical role of TNF-α in immune cell-induced intestinal glucocorticoid synthesis.—Noti, M., Corazza, N., Tuffin, G., Schoonjans, K., Brunner, T. Lipopolysaccharide induces intestinal glucocorticoid synthesis in a TNFα-dependent manner.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
inflammatory bowel disease,tumor necrosis factor α,intestinal epithelial cells,macrophages,TNF receptors
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
NOTI, Mario, Nadia CORAZZA, Gèrald TUFFIN, Kristina SCHOONJANS, Thomas BRUNNER, 2010. Lipopolysaccharide induces intestinal glucocorticoid synthesis in a TNFalpha-dependent manner. In: The FASEB Journal. 24(5), pp. 1340-1346. ISSN 0892-6638. eISSN 1530-6860. Available under: doi: 10.1096/fj.09-140913BibTex
@article{Noti2010-05Lipop-13536, year={2010}, doi={10.1096/fj.09-140913}, title={Lipopolysaccharide induces intestinal glucocorticoid synthesis in a TNFalpha-dependent manner}, number={5}, volume={24}, issn={0892-6638}, journal={The FASEB Journal}, pages={1340--1346}, author={Noti, Mario and Corazza, Nadia and Tuffin, Gèrald and Schoonjans, Kristina and Brunner, Thomas} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/13536"> <dcterms:title>Lipopolysaccharide induces intestinal glucocorticoid synthesis in a TNFalpha-dependent manner</dcterms:title> <dc:creator>Tuffin, Gèrald</dc:creator> <dc:creator>Schoonjans, Kristina</dc:creator> <dcterms:abstract xml:lang="eng">Stringent control of immune responses in the intestinal mucosa is critical for the maintenance of immune homeostasis and prevention of tissue damage, such as observed during inflammatory bowel disease. Intestinal epithelial cells, primarily thought to form a simple physical barrier, critically regulate intestinal immune cell functions by producing immunoregulatory glucocorticoids on T-cell activation. In this study we investigated whether stimulation of cells of the innate immune system results in the induction of intestinal glucocorticoids synthesis and what role TNF-α plays in this process. Stimulation of the innate immune system with lipopolysaccharide (LPS) led to an up-regulation of colonic steroidogenic enzymes and the induction of intestinal glucocorticoid synthesis. The observed induction was dependent on macrophage effector functions, as depletion of macrophages using clodronate-containing liposomes, but not absence of T and B cells, inhibited intestinal glucocorticoid synthesis. LPS-induced glucocorticoid synthesis was critically dependent on TNF-α as it was significantly decreased in TNF-α-deficient animals. Both TNF receptor-1 and -2 were found to be equally involved in LPS- and T-cell-induced intestinal GC synthesis. These results describe a novel and critical role of TNF-α in immune cell-induced intestinal glucocorticoid synthesis.—Noti, M., Corazza, N., Tuffin, G., Schoonjans, K., Brunner, T. Lipopolysaccharide induces intestinal glucocorticoid synthesis in a TNFα-dependent manner.</dcterms:abstract> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/13536/2/2010_Lipopolysach.pdf"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/13536"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:creator>Noti, Mario</dc:creator> <dc:creator>Corazza, Nadia</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Tuffin, Gèrald</dc:contributor> <dc:contributor>Noti, Mario</dc:contributor> <dc:creator>Brunner, Thomas</dc:creator> <dcterms:bibliographicCitation>First publ. in: The FASEB Journal ; 24 (2010), 5. - S. 1340-1346</dcterms:bibliographicCitation> <dc:contributor>Schoonjans, Kristina</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-08-30T22:25:05Z</dcterms:available> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/13536/2/2010_Lipopolysach.pdf"/> <dc:contributor>Corazza, Nadia</dc:contributor> <dc:language>eng</dc:language> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-05-31T09:44:30Z</dc:date> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:issued>2010-05</dcterms:issued> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Brunner, Thomas</dc:contributor> </rdf:Description> </rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
Yes