Dynamics of BMP signaling and distribution during zebrafish dorsal-ventral patterning

Pomreinke, Autumn P.; Soh, Gary H.; Rogers, Katherine W.; Bergmann, Jennifer K.; Bläßle, Alexander J.; Müller, Patrick

Dynamics of BMP signaling and distribution during zebrafish dorsal-ventral patterning

Dateien

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Datum

2017

Autor:innen

Pomreinke, Autumn P.

Soh, Gary H.

Rogers, Katherine W.

Bergmann, Jennifer K.

Bläßle, Alexander J.

Müller, Patrick

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

eLife. eLife Sciences Publications. 2017, 6, e25861. eISSN 2050-084X. Available under: doi: 10.7554/eLife.25861

Zusammenfassung

During vertebrate embryogenesis, dorsal-ventral patterning is controlled by the BMP/Chordin activator/inhibitor system. BMP induces ventral fates, whereas Chordin inhibits BMP signaling on the dorsal side. Several theories can explain how the distributions of BMP and Chordin are regulated to achieve patterning, but the assumptions regarding activator/inhibitor diffusion and stability differ between models. Notably, 'shuttling' models in which the BMP distribution is modulated by a Chordin-mediated increase in BMP diffusivity have gained recent prominence. Here, we directly test five major models by measuring the biophysical properties of fluorescently tagged BMP2b and Chordin in zebrafish embryos. We found that BMP2b and Chordin diffuse and rapidly form extracellular protein gradients, Chordin does not modulate the diffusivity or distribution of BMP2b, and Chordin is not required to establish peak levels of BMP signaling. Our findings challenge current self-regulating reaction-diffusion and shuttling models and provide support for a graded source-sink mechanism underlying zebrafish dorsal-ventral patterning.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

POMREINKE, Autumn P., Gary H. SOH, Katherine W. ROGERS, Jennifer K. BERGMANN, Alexander J. BLÄSSLE, Patrick MÜLLER, 2017. Dynamics of BMP signaling and distribution during zebrafish dorsal-ventral patterning. In: eLife. eLife Sciences Publications. 2017, 6, e25861. eISSN 2050-084X. Available under: doi: 10.7554/eLife.25861

BibTex

@article{Pomreinke2017Dynam-56090,
  year={2017},
  doi={10.7554/eLife.25861},
  title={Dynamics of BMP signaling and distribution during zebrafish dorsal-ventral patterning},
  volume={6},
  journal={eLife},
  author={Pomreinke, Autumn P. and Soh, Gary H. and Rogers, Katherine W. and Bergmann, Jennifer K. and Bläßle, Alexander J. and Müller, Patrick},
  note={Article Number: e25861}
}

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