Birefringence and elasticity of overstretched DNA
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
The conformation of DNA when it interacts with enzymes is not yet well known. To find out more about it and the overstretched state during the process several methods can be applied. One of them is to measure the birefringence of an artificially overstretched molecule. This matter is discussed in the following chapters. Parts of the experiments are the chemical treatment of the DNA to attach it to a surface, using a Surface Force Apparatus, focusing a laser to pass DNA within 10 µm, fluorescent microscopy, electrophoresis and to use supermagnetic beads to manipulate the molecules. All these steps are briefly described.
We were able to measure birefringence of stretched DNA, but not the birefringence of overstretched DNA. As a results of the research we gained a lot of information about the techniques and the birefringence measurement. This report of research gives an idea of the problems and possible solutions of those processes.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
STRACK, Florian, 1999. Birefringence and elasticity of overstretched DNA [Master thesis]BibTex
@mastersthesis{Strack1999Biref-4696,
year={1999},
title={Birefringence and elasticity of overstretched DNA},
author={Strack, Florian}
}RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/4696">
<dc:format>application/pdf</dc:format>
<dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/4696/1/449_1.pdf"/>
<dc:language>eng</dc:language>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/41"/>
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dc:contributor>Strack, Florian</dc:contributor>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/41"/>
<dcterms:issued>1999</dcterms:issued>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T14:49:39Z</dc:date>
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T14:49:39Z</dcterms:available>
<dcterms:title>Birefringence and elasticity of overstretched DNA</dcterms:title>
<dc:creator>Strack, Florian</dc:creator>
<dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/4696/1/449_1.pdf"/>
<bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/4696"/>
<dc:rights>terms-of-use</dc:rights>
<dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
<dcterms:abstract xml:lang="eng">The conformation of DNA when it interacts with enzymes is not yet well known. To find out more about it and the overstretched state during the process several methods can be applied. One of them is to measure the birefringence of an artificially overstretched molecule. This matter is discussed in the following chapters. Parts of the experiments are the chemical treatment of the DNA to attach it to a surface, using a Surface Force Apparatus, focusing a laser to pass DNA within 10 µm, fluorescent microscopy, electrophoresis and to use supermagnetic beads to manipulate the molecules. All these steps are briefly described.<br />We were able to measure birefringence of stretched DNA, but not the birefringence of overstretched DNA. As a results of the research we gained a lot of information about the techniques and the birefringence measurement. This report of research gives an idea of the problems and possible solutions of those processes.</dcterms:abstract>
</rdf:Description>
</rdf:RDF>
