Birefringence and elasticity of overstretched DNA
Birefringence and elasticity of overstretched DNA
Loading...
Files
Date
1999
Authors
Strack, Florian
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Title in another language
Publication type
Diploma thesis
Publication status
Published in
Abstract
The conformation of DNA when it interacts with enzymes is not yet well known. To find out more about it and the overstretched state during the process several methods can be applied. One of them is to measure the birefringence of an artificially overstretched molecule. This matter is discussed in the following chapters. Parts of the experiments are the chemical treatment of the DNA to attach it to a surface, using a Surface Force Apparatus, focusing a laser to pass DNA within 10 µm, fluorescent microscopy, electrophoresis and to use supermagnetic beads to manipulate the molecules. All these steps are briefly described.
We were able to measure birefringence of stretched DNA, but not the birefringence of overstretched DNA. As a results of the research we gained a lot of information about the techniques and the birefringence measurement. This report of research gives an idea of the problems and possible solutions of those processes.
We were able to measure birefringence of stretched DNA, but not the birefringence of overstretched DNA. As a results of the research we gained a lot of information about the techniques and the birefringence measurement. This report of research gives an idea of the problems and possible solutions of those processes.
Summary in another language
Subject (DDC)
530 Physics
Keywords
DNA
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
STRACK, Florian, 1999. Birefringence and elasticity of overstretched DNA [Master thesis]BibTex
@mastersthesis{Strack1999Biref-4696,
year={1999},
title={Birefringence and elasticity of overstretched DNA},
author={Strack, Florian}
}
RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/4696">
<dc:format>application/pdf</dc:format>
<dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/4696/1/449_1.pdf"/>
<dc:language>eng</dc:language>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/41"/>
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dc:contributor>Strack, Florian</dc:contributor>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/41"/>
<dcterms:issued>1999</dcterms:issued>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T14:49:39Z</dc:date>
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T14:49:39Z</dcterms:available>
<dcterms:title>Birefringence and elasticity of overstretched DNA</dcterms:title>
<dc:creator>Strack, Florian</dc:creator>
<dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/4696/1/449_1.pdf"/>
<bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/4696"/>
<dc:rights>terms-of-use</dc:rights>
<dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
<dcterms:abstract xml:lang="eng">The conformation of DNA when it interacts with enzymes is not yet well known. To find out more about it and the overstretched state during the process several methods can be applied. One of them is to measure the birefringence of an artificially overstretched molecule. This matter is discussed in the following chapters. Parts of the experiments are the chemical treatment of the DNA to attach it to a surface, using a Surface Force Apparatus, focusing a laser to pass DNA within 10 µm, fluorescent microscopy, electrophoresis and to use supermagnetic beads to manipulate the molecules. All these steps are briefly described.<br />We were able to measure birefringence of stretched DNA, but not the birefringence of overstretched DNA. As a results of the research we gained a lot of information about the techniques and the birefringence measurement. This report of research gives an idea of the problems and possible solutions of those processes.</dcterms:abstract>
</rdf:Description>
</rdf:RDF>