PARP1 protects from benzo[a]pyrene diol epoxide-induced replication stress and mutagenicity

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Zusammenfassung

Poly(ADP-ribosyl)ation (PARylation) is a complex and reversible posttranslational modification catalyzed by poly(ADP-ribose)polymerases (PARPs), which orchestrates protein function and subcellular localization. The function of PARP1 in genotoxic stress response upon induction of oxidative DNA lesions and strand breaks is firmly established, but its role in the response to chemical-induced, bulky DNA adducts is understood incompletely. To address the role of PARP1 in the response to bulky DNA adducts, we treated human cancer cells with benzo[a]pyrene 7,8-dihydrodiol-9,10-epoxide (BPDE), which represents the active metabolite of the environmental carcinogen benzo[a]pyrene [B(a)P], in nanomolar to low micromolar concentrations. Using a highly sensitive LC-MS/MS method, we revealed that BPDE induces cellular PAR formation in a time- and dose-dependent manner. Consistently, PARP1 activity significantly contributed to BPDE-induced genotoxic stress response. On one hand, PARP1 ablation rescued BPDE-induced NAD+ depletion and protected cells from BPDE-induced short-term toxicity. On the other hand, strong sensitization effects of PARP inhibition and PARP1 ablation were observed in long-term clonogenic survival assays. Furthermore, PARP1 ablation significantly affected BPDE-induced S- and G2-phase transitions. Together, these results point towards unresolved BPDE-DNA lesions triggering replicative stress. In line with this, BPDE exposure resulted in enhanced formation and persistence of DNA double-strand breaks in PARP1-deficient cells as evaluated by microscopic co-localization studies of 53BP1 and γH2A.X foci. Consistently, an HPRT mutation assay revealed that PARP inhibition potentiated the mutagenicity of BPDE. In conclusion, this study demonstrates a profound role of PARylation in BPDE-induced genotoxic stress response with significant functional consequences and potential relevance with regard to B[a]P-induced cancer risks.

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570 Biowissenschaften, Biologie
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PARP 1, Poly(ADP-ribosyl)ation, Benzo[a]pyrene, BPDE, Replication stress
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ISO 690FISCHER, Jan M.F., Tabea ZUBEL, Kirsten JANDER, Jelena FIX, Irmela R.E.A. TRUSSINA, Daniel GEBHARD, Jörg BERGEMANN, Alexander BÜRKLE, Aswin MANGERICH, 2018. PARP1 protects from benzo[a]pyrene diol epoxide-induced replication stress and mutagenicity. In: Archives of toxicology. 2018, 92(3), pp. 1323-1340. ISSN 0340-5761. eISSN 1432-0738. Available under: doi: 10.1007/s00204-017-2115-6
BibTex
@article{Fischer2018-03PARP1-40871,
  year={2018},
  doi={10.1007/s00204-017-2115-6},
  title={PARP1 protects from benzo[a]pyrene diol epoxide-induced replication stress and mutagenicity},
  number={3},
  volume={92},
  issn={0340-5761},
  journal={Archives of toxicology},
  pages={1323--1340},
  author={Fischer, Jan M.F. and Zubel, Tabea and Jander, Kirsten and Fix, Jelena and Trussina, Irmela R.E.A. and Gebhard, Daniel and Bergemann, Jörg and Bürkle, Alexander and Mangerich, Aswin}
}
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