@article{Perlman2005-01Highc-14049,
  year={2005},
  doi={10.1002/cbic.200400266},
  title={High-content screening and profiling of drug activity in an automated centrosome-duplication assa},
  number={1},
  volume={6},
  issn={1439-4227},
  journal={ChemBioChem},
  pages={145--151},
  author={Perlman, Zachary E. and Mitchison, Timothy J. and Mayer, Thomas U.}
}

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    <dcterms:abstract xml:lang="eng">Maintenance of centrosome number is essential for cell-cycle progression and genomic stability, but investigation of this regulation has been limited by assay difficulty. We present a fully automated image-based centrosome-duplication assay that is accurate and robust enough for both careful cell-biology studies and high-throughput screening, and employ this assay in a series of chemical-genetic studies. We observe that a simple cytometric profiling strategy, which is based on organelle size, groups compounds with similar mechanisms of action; this suggests a simple strategy for excluding compounds that undesirably target such activities as protein synthesis and microtubule dynamics. Screening a library of compounds of known activity, we found unexpected effects on centrosome duplication by a number of drugs, most notably isoform-specific protein kinase C inhibitors and retinoic acid receptor agonists. From a 16 320-member library of uncharacterized small molecules, we identified five potent centrosome-duplication inhibitors that do not target microtubule dynamics or protein synthesis. The analysis methodology reported here is directly relevant to studies of centrosome regulation in a variety of systems and is adaptable to a wide range of other biological problems.</dcterms:abstract>
    <dc:contributor>Mayer, Thomas U.</dc:contributor>
    <dcterms:title>High-content screening and profiling of drug activity in an automated centrosome-duplication assa</dcterms:title>
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    <dc:creator>Mitchison, Timothy J.</dc:creator>
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    <dc:creator>Perlman, Zachary E.</dc:creator>
    <dc:contributor>Mitchison, Timothy J.</dc:contributor>
    <dcterms:issued>2005-01</dcterms:issued>
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    <dc:contributor>Perlman, Zachary E.</dc:contributor>
    <dc:creator>Mayer, Thomas U.</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:bibliographicCitation>Publ. in: Chembiochem ; 6 (2005), 1. - S. 145-151</dcterms:bibliographicCitation>
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