Exploration of the TRIM Fold of MuRF1 Using EPR Reveals a Canonical Antiparallel Structure and Extended COS-Box

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2019
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Stevens, Michael
Skorupka, Katarzyna A.
Cafiso, David S.
Pornillos, Owen
Norman, David G.
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Journal of molecular biology. 2019, 431(15), pp. 2900-2909. ISSN 0022-2836. eISSN 1089-8638. Available under: doi: 10.1016/j.jmb.2019.05.025
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MuRF1 (TRIM63) is a RING-type E3 ubiquitin ligase with a predicted tripartite TRIM fold. TRIM proteins rely upon the correct placement of an N-terminal RING domain, with respect to C-terminal, specific substrate-binding domains. The TRIM domain organization is orchestrated by a central helical domain that forms an antiparallel coiled-coil motif and mediates the dimerization of the fold. MuRF1 has a reduced TRIM composition characterized by a lack of specific substrate binding domains, but contains in its helical domain a conserved sequence motif termed COS-box that has been speculated to fold independently into an α-hairpin. These characteristics had led to question whether MuRF1 adopts a canonical TRIM fold. Using a combination of electron paramagnetic resonance, on spin-labeled protein, and disulfide crosslinking, we show that TRIM63 follows the structural conservation of the TRIM dimerization domain, observed in other proteins. We also show that the COS-box motif folds back onto the dimerization coiled-coil motif, predictably forming a four-helical bundle at the center of the protein and emulating the architecture of canonical TRIMs.

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ISO 690STEVENS, Michael, Barbara FRANKE, Katarzyna A. SKORUPKA, David S. CAFISO, Owen PORNILLOS, Olga MAYANS, David G. NORMAN, 2019. Exploration of the TRIM Fold of MuRF1 Using EPR Reveals a Canonical Antiparallel Structure and Extended COS-Box. In: Journal of molecular biology. 2019, 431(15), pp. 2900-2909. ISSN 0022-2836. eISSN 1089-8638. Available under: doi: 10.1016/j.jmb.2019.05.025
BibTex
@article{Stevens2019-07-12Explo-46660,
  year={2019},
  doi={10.1016/j.jmb.2019.05.025},
  title={Exploration of the TRIM Fold of MuRF1 Using EPR Reveals a Canonical Antiparallel Structure and Extended COS-Box},
  number={15},
  volume={431},
  issn={0022-2836},
  journal={Journal of molecular biology},
  pages={2900--2909},
  author={Stevens, Michael and Franke, Barbara and Skorupka, Katarzyna A. and Cafiso, David  S. and Pornillos, Owen and Mayans, Olga and Norman, David G.}
}
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