A broadband waveguide for protein crystallography under intense microwave fields
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We present a slab-line waveguide whose geometry is optimized for wide-angle x-ray diffraction (XRD) experiments on protein crystals during irradiation with intense microwave fields. Characterization of the waveguide transmission and reflectivity (using time-domain reflectometry) and of the electric field distribution inside the waveguide (using finite-difference time-domain calculations) shows that the present device has a broad bandwidth from below 0.5 to 18 GHz, allowing one to perform frequency-dependent XRD studies with a well-defined transverse mode structure and negligible reflection losses. As shown with a specific example, our device provides a simple way to couple microwave irradiation experiments with high-resolution x-ray diffraction measurements from millimeter-size crystalline samples. The present design might prove useful for systematic studies of microwave effects on protein structure and dynamics.
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WEISSENBORN, Ralf, Tina REINHARDT, Volkert HANSEN, Georg MARET, Thomas GISLER, 2004. A broadband waveguide for protein crystallography under intense microwave fields. In: Review of Scientific Instruments. 2004, 75(12), pp. 5253-5256. Available under: doi: 10.1063/1.1813771BibTex
@article{Weissenborn2004broad-5301, year={2004}, doi={10.1063/1.1813771}, title={A broadband waveguide for protein crystallography under intense microwave fields}, number={12}, volume={75}, journal={Review of Scientific Instruments}, pages={5253--5256}, author={Weissenborn, Ralf and Reinhardt, Tina and Hansen, Volkert and Maret, Georg and Gisler, Thomas} }
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