Publikation: Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide
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2020
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Chemistry. MDPI. 2020, 2(2), pp. 490-498. eISSN 2624-8549. Available under: doi: 10.3390/chemistry2020029
Zusammenfassung
LAMP is an approach for isothermal nucleic acids diagnostics with increasing importance but suffers from the need of tedious systems design and optimization for every new target. Here, we describe an approach for its simplification based on a single nucleoside-5′-O-triphosphate (dNTP) that is covalently modified with a DNA strand. We found that the DNA-modified dNTP is a substrate for DNA polymerases in versatile primer extension reactions despite its size and that the incorporated DNA indeed serves as a target for selective LAMP analysis.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
modified nucleotide; DNA polymerase; LAMP; primer extension
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WELTER, Moritz, Andreas MARX, 2020. Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide. In: Chemistry. MDPI. 2020, 2(2), pp. 490-498. eISSN 2624-8549. Available under: doi: 10.3390/chemistry2020029BibTex
@article{Welter2020-06Combi-52032,
year={2020},
doi={10.3390/chemistry2020029},
title={Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide},
number={2},
volume={2},
journal={Chemistry},
pages={490--498},
author={Welter, Moritz and Marx, Andreas}
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