Integrin-mediated internalization of Staphylococcus aureus does not require vinculin
Integrin-mediated internalization of Staphylococcus aureus does not require vinculin
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BMC Cell Biology ; 14 (2013), 1. - 2. - ISSN 1471-2121. - eISSN 1471-2121
Zusammenfassung
Background: Disease manifestations of Staphylococcus aureus are connected to the fibronectin (Fn)-binding
capacity of these Gram-positive pathogens. Fn deposition on the surface of S. aureus allows engagement of α5β1 integrins and triggers uptake by host cells. For several integrin- and actin-associated cytoplasmic proteins, including FAK, Src, N-WASP, tensin and cortactin, a functional role during bacterial invasion has been demonstrated. As
reorganization of the actin cytoskeleton is critical for bacterial entry, we investigated whether vinculin, an essential protein linking integrins with the actin cytoskeleton, may contribute to the integrin-mediated internalization of S. aureus.
Results: Complementation of vinculin in vinculin -/- cells, vinculin overexpression, as well as shRNA-mediated vinculin knock-down in different eukaryotic cell types demonstrate, that vinculin does not have a functional role during the integrin-mediated uptake of S. aureus.
Conclusions: Our results suggest that vinculin is insignificant for the integrin-mediated uptake of S. aureus despite the critical role of vinculin as a linker between integrins and F-actin.
capacity of these Gram-positive pathogens. Fn deposition on the surface of S. aureus allows engagement of α5β1 integrins and triggers uptake by host cells. For several integrin- and actin-associated cytoplasmic proteins, including FAK, Src, N-WASP, tensin and cortactin, a functional role during bacterial invasion has been demonstrated. As
reorganization of the actin cytoskeleton is critical for bacterial entry, we investigated whether vinculin, an essential protein linking integrins with the actin cytoskeleton, may contribute to the integrin-mediated internalization of S. aureus.
Results: Complementation of vinculin in vinculin -/- cells, vinculin overexpression, as well as shRNA-mediated vinculin knock-down in different eukaryotic cell types demonstrate, that vinculin does not have a functional role during the integrin-mediated uptake of S. aureus.
Conclusions: Our results suggest that vinculin is insignificant for the integrin-mediated uptake of S. aureus despite the critical role of vinculin as a linker between integrins and F-actin.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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Staphylococcus aureus,Bacterial adhesion,Endocytosis,Fibronectin,Host cell invasion,Integrin,Vinculin
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BORISOVA, Marina, Yong SHI, Alexander BUNTRU, Susanne WÖRNER, Wolfgang H. ZIEGLER, Christof R. HAUCK, 2013. Integrin-mediated internalization of Staphylococcus aureus does not require vinculin. In: BMC Cell Biology. 14(1), 2. ISSN 1471-2121. eISSN 1471-2121. Available under: doi: 10.1186/1471-2121-14-2BibTex
@article{Borisova2013Integ-21823, year={2013}, doi={10.1186/1471-2121-14-2}, title={Integrin-mediated internalization of Staphylococcus aureus does not require vinculin}, number={1}, volume={14}, issn={1471-2121}, journal={BMC Cell Biology}, author={Borisova, Marina and Shi, Yong and Buntru, Alexander and Wörner, Susanne and Ziegler, Wolfgang H. and Hauck, Christof R.}, note={Article Number: 2} }
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