Activation of p53 by conjugation to the ubiquitin‐like protein SUMO‐1

Gotissa, Monica; Hengstermann, Arnd; Fogal, Valentina; Sandy, Peter; Schwarz, Sylvia E.; Scheffner, Martin; Del Sal, Giannino

Activation of p53 by conjugation to the ubiquitin‐like protein SUMO‐1

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

1999

Autor:innen

Gotissa, Monica

Hengstermann, Arnd

Fogal, Valentina

Sandy, Peter

Schwarz, Sylvia E.

Scheffner, Martin

Del Sal, Giannino

DOI (zitierfähiger Link)

10.1093/emboj/18.22.6462

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

The EMBO Journal ; 18 (1999), 22. - S. 6462-6471. - ISSN 0261-4189. - eISSN 1460-2075

Zusammenfassung

The growth-suppressive properties of p53 are controlled by posttranslational modifications and by regulation of its turnover rate. Here we show that p53 can be modified in vitro and in vivo by conjugation to the small ubiquitin-like protein SUMO-1. A lysine residue at amino acid position 386 of p53 is required for this previously undescribed modification, strongly suggesting that this lysine residue serves as the major attachment site for SUMO-1. Unlike ubiquitin, attachment of SUMO-1 does not appear to target proteins for rapid degradation but rather, has been proposed to change the ability of the modified protein to interact with other cellular proteins. Accordingly, we provide evidence that conjugation of SUMO-1 to wild-type p53 results in an increased transactivation ability of p53. We suggest that posttranslational modification of p53 by SUMO-1 conjugation provides a novel mechanism to regulate p53 activity.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

p53; regulation; SUMO-1; transactivation; Ubc9

Zitieren

ISO 690

GOTISSA, Monica, Arnd HENGSTERMANN, Valentina FOGAL, Peter SANDY, Sylvia E. SCHWARZ, Martin SCHEFFNER, Giannino DEL SAL, 1999. Activation of p53 by conjugation to the ubiquitin‐like protein SUMO‐1. In: The EMBO Journal. 18(22), pp. 6462-6471. ISSN 0261-4189. eISSN 1460-2075. Available under: doi: 10.1093/emboj/18.22.6462

BibTex

@article{Gotissa1999-11-15Activ-42656,
  year={1999},
  doi={10.1093/emboj/18.22.6462},
  title={Activation of p53 by conjugation to the ubiquitin‐like protein SUMO‐1},
  number={22},
  volume={18},
  issn={0261-4189},
  journal={The EMBO Journal},
  pages={6462--6471},
  author={Gotissa, Monica and Hengstermann, Arnd and Fogal, Valentina and Sandy, Peter and Schwarz, Sylvia E. and Scheffner, Martin and Del Sal, Giannino}
}

RDF

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