Zonal rotor centrifugation revisited : new horizons in sorting nanoparticles
Dateien
Datum
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
Density gradient centrifugation is an effective method for the isolation and purification of small particles. Hollow rotors capable of hosting density gradients replace the need for centrifuge tubes and therefore allow separations at large scales. So far, zonal rotors have been used for biological separations ranging from the purification of whole cells down to serum proteins. We demonstrate that the high-resolution separation method opens up exciting perspectives apart from biology, namely in sorting mixtures of synthetic nanoparticles. Loading and unloading, while the rotor is spinning, avoids perturbations during acceleration and deceleration periods, and thus makes a vital contribution to sorting accuracy. Nowadays one can synthesize nanoscale particles in a wide variety of compositions and shapes. A prominent example for this are “colloidal molecules” or, generally speaking, defined assemblies of nanoparticles that can appear in varying aggregation numbers. Fractionation of such multimodal colloids plays an essential role with regard to their organization into hierarchical organized superstructures such as films, mesocrystals and metamaterials. Zonal rotor centrifugation was found to be a scalable method of getting “colloidal molecules” properly sorted. It allows access to pure fractions of particle monomers, dimers, and trimers, just as well as to fractions that are essentially rich in particle tetramers. Separations were evaluated by differential centrifugal sedimentation, which provides high-resolution size distributions of the collected nanoparticle fractions. The performance achieved in relation to resolution, zone widths, sorting efficiencies and recovery rates clearly demonstrate that zonal rotor centrifugation provides an excellent solution to the fractionation of nanoparticle mixtures.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
PLÜISCH, Claudia Simone, Brigitte BÖSSENECKER, Lukas DOBLER, Alexander WITTEMANN, 2019. Zonal rotor centrifugation revisited : new horizons in sorting nanoparticles. In: RSC Advances. 2019, 9(47), pp. 27549-27559. eISSN 2046-2069. Available under: doi: 10.1039/C9RA05140FBibTex
@article{Pluisch2019-09-02Zonal-46801, year={2019}, doi={10.1039/C9RA05140F}, title={Zonal rotor centrifugation revisited : new horizons in sorting nanoparticles}, number={47}, volume={9}, journal={RSC Advances}, pages={27549--27559}, author={Plüisch, Claudia Simone and Bössenecker, Brigitte and Dobler, Lukas and Wittemann, Alexander} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/46801"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-09-09T11:57:21Z</dc:date> <dc:creator>Bössenecker, Brigitte</dc:creator> <dc:contributor>Plüisch, Claudia Simone</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/46801"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dcterms:abstract xml:lang="eng">Density gradient centrifugation is an effective method for the isolation and purification of small particles. Hollow rotors capable of hosting density gradients replace the need for centrifuge tubes and therefore allow separations at large scales. So far, zonal rotors have been used for biological separations ranging from the purification of whole cells down to serum proteins. We demonstrate that the high-resolution separation method opens up exciting perspectives apart from biology, namely in sorting mixtures of synthetic nanoparticles. Loading and unloading, while the rotor is spinning, avoids perturbations during acceleration and deceleration periods, and thus makes a vital contribution to sorting accuracy. Nowadays one can synthesize nanoscale particles in a wide variety of compositions and shapes. A prominent example for this are “colloidal molecules” or, generally speaking, defined assemblies of nanoparticles that can appear in varying aggregation numbers. Fractionation of such multimodal colloids plays an essential role with regard to their organization into hierarchical organized superstructures such as films, mesocrystals and metamaterials. Zonal rotor centrifugation was found to be a scalable method of getting “colloidal molecules” properly sorted. It allows access to pure fractions of particle monomers, dimers, and trimers, just as well as to fractions that are essentially rich in particle tetramers. Separations were evaluated by differential centrifugal sedimentation, which provides high-resolution size distributions of the collected nanoparticle fractions. The performance achieved in relation to resolution, zone widths, sorting efficiencies and recovery rates clearly demonstrate that zonal rotor centrifugation provides an excellent solution to the fractionation of nanoparticle mixtures.</dcterms:abstract> <dc:creator>Wittemann, Alexander</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/46801/5/Plueisch_2-q285kpqrzt5a8.pdf"/> <dc:creator>Dobler, Lukas</dc:creator> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/3.0/"/> <dc:creator>Plüisch, Claudia Simone</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:contributor>Bössenecker, Brigitte</dc:contributor> <dc:contributor>Wittemann, Alexander</dc:contributor> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/46801/5/Plueisch_2-q285kpqrzt5a8.pdf"/> <dc:rights>Attribution 3.0 Unported</dc:rights> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-09-09T11:57:21Z</dcterms:available> <dcterms:issued>2019-09-02</dcterms:issued> <dc:language>eng</dc:language> <dc:contributor>Dobler, Lukas</dc:contributor> <dcterms:title>Zonal rotor centrifugation revisited : new horizons in sorting nanoparticles</dcterms:title> </rdf:Description> </rdf:RDF>