Publikation: PrPc and reggies/flotillins are contained in and released via lipid-rich vesicles in Jurkat T cells
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2004
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Cellular and Molecular Life Sciences. 2004, 61(16), pp. 2092-2099. ISSN 1420-682X. eISSN 1420-9071. Available under: doi: 10.1007/s00018-004-4193-x
Zusammenfassung
A new model of caveolin association with lipid body cores has recently been proposed which may be relevant to a number of cellular processes, e.g. lipid body generation. Here we show that PrPc and reggie-1 and reggie-2 also occur in the cores of Nile Red/Bodipy-stained (neutral lipid-containing) vesicular structures and, in immunoblots, in the lipid-enriched fraction after density gradient centrifugation. These lipid-rich vesicles increase in number following cell feeding with oleic acid, differ from early endosome antigen 1- and Lamp-2-positive endosomes/lysosomes, exhibit an opaque content and lack surrounding actin staining. Our results suggest that the content of these vesicles, together with reggie-1 and -2 and PrPc, is expelled.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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Cellular prion protein, reggie/flotillin, lipid-rich vesicle, release/exocytosis
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REUTER, Alexander, Ulrike BINKLE, Claudia STÜRMER, Helmut PLATTNER, 2004. PrPc and reggies/flotillins are contained in and released via lipid-rich vesicles in Jurkat T cells. In: Cellular and Molecular Life Sciences. 2004, 61(16), pp. 2092-2099. ISSN 1420-682X. eISSN 1420-9071. Available under: doi: 10.1007/s00018-004-4193-xBibTex
@article{Reuter2004reggi-7056,
year={2004},
doi={10.1007/s00018-004-4193-x},
title={PrPc and reggies/flotillins are contained in and released via lipid-rich vesicles in Jurkat T cells},
number={16},
volume={61},
issn={1420-682X},
journal={Cellular and Molecular Life Sciences},
pages={2092--2099},
author={Reuter, Alexander and Binkle, Ulrike and Stürmer, Claudia and Plattner, Helmut}
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