The yeast ERAD-C ubiquitin ligase Doa10 recognizes an intramembrane degron

Habeck, Gregor; Ebner, Felix A.; Shimada-Kreft, Hiroko; Kreft, Stefan G.

The yeast ERAD-C ubiquitin ligase Doa10 recognizes an intramembrane degron

Dateien

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Datum

2015

Autor:innen

Habeck, Gregor

Ebner, Felix A.

Shimada-Kreft, Hiroko

Kreft, Stefan G.

DOI (zitierfähiger Link)

https://doi.org/10.1083/jcb.201408088

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

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The Journal of Cell Biology. 2015, 209(2), pp. 261-273. ISSN 0021-9525. eISSN 1540-8140. Available under: doi: 10.1083/jcb.201408088

Zusammenfassung

Aberrant endoplasmic reticulum (ER) proteins are eliminated by ER-associated degradation (ERAD). This process involves protein retrotranslocation into the cytosol, ubiquitylation, and proteasomal degradation. ERAD substrates are classified into three categories based on the location of their degradation signal/degron: ERAD-L (lumen), ERAD-M (membrane), and ERAD-C (cytosol) substrates. In Saccharomyces cerevisiae, the membrane proteins Hrd1 and Doa10 are the predominant ERAD ubiquitin-protein ligases (E3s). The current notion is that ERAD-L and ERAD-M substrates are exclusively handled by Hrd1, whereas ERAD-C substrates are recognized by Doa10. In this paper, we identify the transmembrane (TM) protein Sec61 β-subunit homologue 2 (Sbh2) as a Doa10 substrate. Sbh2 is part of the trimeric Ssh1 complex involved in protein translocation. Unassembled Sbh2 is rapidly degraded in a Doa10-dependent manner. Intriguingly, the degron maps to the Sbh2 TM region. Thus, in contrast to the prevailing view, Doa10 (and presumably its human orthologue) has the capacity for recognizing intramembrane degrons, expanding its spectrum of substrates.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

HABECK, Gregor, Felix A. EBNER, Hiroko SHIMADA-KREFT, Stefan G. KREFT, 2015. The yeast ERAD-C ubiquitin ligase Doa10 recognizes an intramembrane degron. In: The Journal of Cell Biology. 2015, 209(2), pp. 261-273. ISSN 0021-9525. eISSN 1540-8140. Available under: doi: 10.1083/jcb.201408088

BibTex

@article{Habeck2015yeast-31566,
  year={2015},
  doi={10.1083/jcb.201408088},
  title={The yeast ERAD-C ubiquitin ligase Doa10 recognizes an intramembrane degron},
  number={2},
  volume={209},
  issn={0021-9525},
  journal={The Journal of Cell Biology},
  pages={261--273},
  author={Habeck, Gregor and Ebner, Felix A. and Shimada-Kreft, Hiroko and Kreft, Stefan G.}
}

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