Membrane and raft association of reggie-1/flotillin-2 : role of myristoylation, palmitoylation and oligomerization and induction of filopodia by overexpression

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103_Membrane_and_raft_association.pdf
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2004
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Neumann-Giesen, Carolin
Falkenbach, Bianca
Beicht, Peter
Claasen, Stephanie
Lüers, Georg
Herzog, Volker
Tikkanen, Ritva
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Biochemical Journal. 2004, 378(2), pp. 509-518. ISSN 0264-6021. eISSN 1470-8728. Available under: doi: 10.1042/bj20031100
Zusammenfassung

The reggie protein family consists of two proteins, reggie-1 and -2, also called flotillins, which are highly ubiquitous and evolutionarily conserved. Both reggies have been shown to be associated with membrane rafts and are involved in various cellular processes such as T-cell activation, phagocytosis and insulin signalling. However, the exact molecular function of these proteins remains to be determined. In addition, the mechanism of membrane association of reggie-1, which does not contain any transmembrane domain, is not known. In this study, we have produced a fusion protein of reggie-1 with enhanced green fluorescent protein and generated targeted substitutions for the inactivation of putative palmitoylation and myristoylation sites. We were able to show that reggie-1 is myristoylated and multiply palmitoylated and that lipid modifications are necessary for membrane association of reggie-1. Overexpression of reggie-1 resulted in the induction of numerous filopodia-like protrusions in various cell lines, suggesting a role for reggie-1 as a signalling protein in actin-dependent processes.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
actin cytoskeleton, filopodia, flotilin, protein acylation, rafts, reggie
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ISO 690NEUMANN-GIESEN, Carolin, Bianca FALKENBACH, Peter BEICHT, Stephanie CLAASEN, Georg LÜERS, Claudia STÜRMER, Volker HERZOG, Ritva TIKKANEN, 2004. Membrane and raft association of reggie-1/flotillin-2 : role of myristoylation, palmitoylation and oligomerization and induction of filopodia by overexpression. In: Biochemical Journal. 2004, 378(2), pp. 509-518. ISSN 0264-6021. eISSN 1470-8728. Available under: doi: 10.1042/bj20031100
BibTex
@article{NeumannGiesen2004Membr-8611,
  year={2004},
  doi={10.1042/bj20031100},
  title={Membrane and raft association of reggie-1/flotillin-2 : role of myristoylation, palmitoylation and oligomerization and induction of filopodia by overexpression},
  number={2},
  volume={378},
  issn={0264-6021},
  journal={Biochemical Journal},
  pages={509--518},
  author={Neumann-Giesen, Carolin and Falkenbach, Bianca and Beicht, Peter and Claasen, Stephanie and Lüers, Georg and Stürmer, Claudia and Herzog, Volker and Tikkanen, Ritva}
}
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