YbiB from Escherichia coli, the Defining Member of the Novel TrpD2 Family of Prokaryotic DNA-binding Proteins

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2015
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Kaiser, Wolfgang
Stutz, Cian
Holinski, Alexandra
Babinger, Patrick
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We present the crystal structure and biochemical characterization of Escherichia coli YbiB, a member of the hitherto uncharacterized TrpD2 protein family. Our results demonstrate that the functional diversity of proteins with a common fold can be far greater than predictable by computational annotation. The TrpD2 proteins show high structural homology to anthranilate phosphoribosyltransferase (TrpD) and nucleoside phosphorylase class II enzymes but bind with high affinity (KD = 10-100 nM) to nucleic acids without detectable sequence specificity. The difference in affinity between single- and double-stranded DNA is minor. Results suggest that multiple YbiB molecules bind to one longer DNA molecule in a cooperative manner. The YbiB protein is a homodimer that, therefore, has two electropositive DNA binding grooves. But due to negative cooperativity within the dimer, only one groove binds DNA in in vitro experiments. A monomerized variant remains able to bind DNA with similar affinity, but the negative cooperative effect is eliminated. The ybiB gene forms an operon with the DNA helicase gene dinG and is under LexA control, being induced by DNA-damaging agents. Thus, speculatively, the TrpD2 proteins may be part of the LexA-controlled SOS response in bacteria.

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570 Biowissenschaften, Biologie
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ISO 690SCHNEIDER, Daniel, Wolfgang KAISER, Cian STUTZ, Alexandra HOLINSKI, Olga MAYANS, Patrick BABINGER, 2015. YbiB from Escherichia coli, the Defining Member of the Novel TrpD2 Family of Prokaryotic DNA-binding Proteins. In: Journal of Biological Chemistry. 2015, 290(32), pp. 19527-19539. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M114.620575
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@article{Schneider2015-08-07Esche-34938,
  year={2015},
  doi={10.1074/jbc.M114.620575},
  title={YbiB from Escherichia coli, the Defining Member of the Novel TrpD2 Family of Prokaryotic DNA-binding Proteins},
  number={32},
  volume={290},
  issn={0021-9258},
  journal={Journal of Biological Chemistry},
  pages={19527--19539},
  author={Schneider, Daniel and Kaiser, Wolfgang and Stutz, Cian and Holinski, Alexandra and Mayans, Olga and Babinger, Patrick}
}
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