Publikation: Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen
Lade...
Dateien
Datum
1999
Autor:innen
Kapoor, Tarun M.
Haggarty, Stephen J.
King, Randall W.
Schreiber, Stuart L.
Mitchison, Timothy J.
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Science. 1999, 286(5441), pp. 971-974. ISSN 0036-8075. Available under: doi: 10.1126/science.286.5441.971
Zusammenfassung
Small molecules that perturb specific protein functions are valuable tools for dissecting complex processes in mammalian cells. A combination of two phenotype-based screens, one based on a specific posttranslational modification, the other visualizing microtubules and chromatin, was used to identify compounds that affect mitosis. One compound, here named monastrol, arrested mammalian cells in mitosis with monopolar spindles. In vitro, monastrol specifically inhibited the motility of the mitotic kinesin Eg5, a motor protein required for spindle bipolarity. All previously known small molecules that specifically affect the mitotic machinery target tubulin. Monastrol will therefore be a particularly useful tool for studying mitotic mechanisms.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Eg5, checkpoint, spindle, chromosome, Mitose, Chromosomenbande, Zellteilung, Mikrotubuli, Kinesin
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690
MAYER, Thomas U., Tarun M. KAPOOR, Stephen J. HAGGARTY, Randall W. KING, Stuart L. SCHREIBER, Timothy J. MITCHISON, 1999. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. In: Science. 1999, 286(5441), pp. 971-974. ISSN 0036-8075. Available under: doi: 10.1126/science.286.5441.971BibTex
@article{Mayer1999Small-14056,
year={1999},
doi={10.1126/science.286.5441.971},
title={Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen},
number={5441},
volume={286},
issn={0036-8075},
journal={Science},
pages={971--974},
author={Mayer, Thomas U. and Kapoor, Tarun M. and Haggarty, Stephen J. and King, Randall W. and Schreiber, Stuart L. and Mitchison, Timothy J.}
}RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/14056">
<dc:creator>Schreiber, Stuart L.</dc:creator>
<bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14056"/>
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
<dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14056/2/mayer_140561.pdf"/>
<dcterms:issued>1999</dcterms:issued>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-11T11:20:57Z</dcterms:available>
<dc:contributor>Haggarty, Stephen J.</dc:contributor>
<dc:creator>Haggarty, Stephen J.</dc:creator>
<dc:creator>King, Randall W.</dc:creator>
<dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14056/2/mayer_140561.pdf"/>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-11T11:20:57Z</dc:date>
<dc:contributor>King, Randall W.</dc:contributor>
<dc:creator>Mayer, Thomas U.</dc:creator>
<dc:contributor>Schreiber, Stuart L.</dc:contributor>
<dcterms:title>Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen</dcterms:title>
<dc:contributor>Mitchison, Timothy J.</dc:contributor>
<dc:contributor>Mayer, Thomas U.</dc:contributor>
<dc:creator>Mitchison, Timothy J.</dc:creator>
<dcterms:bibliographicCitation>Science ; 286 (1999), 5441. - S. 971-974</dcterms:bibliographicCitation>
<dc:creator>Kapoor, Tarun M.</dc:creator>
<dc:contributor>Kapoor, Tarun M.</dc:contributor>
<dcterms:abstract xml:lang="eng">Small molecules that perturb specific protein functions are valuable tools for dissecting complex processes in mammalian cells. A combination of two phenotype-based screens, one based on a specific posttranslational modification, the other visualizing microtubules and chromatin, was used to identify compounds that affect mitosis. One compound, here named monastrol, arrested mammalian cells in mitosis with monopolar spindles. In vitro, monastrol specifically inhibited the motility of the mitotic kinesin Eg5, a motor protein required for spindle bipolarity. All previously known small molecules that specifically affect the mitotic machinery target tubulin. Monastrol will therefore be a particularly useful tool for studying mitotic mechanisms.</dcterms:abstract>
<dc:rights>terms-of-use</dc:rights>
<dc:language>eng</dc:language>
</rdf:Description>
</rdf:RDF>Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
