Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen

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mayer_140561.pdf
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1999
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Kapoor, Tarun M.
Haggarty, Stephen J.
King, Randall W.
Schreiber, Stuart L.
Mitchison, Timothy J.
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Science. 1999, 286(5441), pp. 971-974. ISSN 0036-8075. Available under: doi: 10.1126/science.286.5441.971
Zusammenfassung

Small molecules that perturb specific protein functions are valuable tools for dissecting complex processes in mammalian cells. A combination of two phenotype-based screens, one based on a specific posttranslational modification, the other visualizing microtubules and chromatin, was used to identify compounds that affect mitosis. One compound, here named monastrol, arrested mammalian cells in mitosis with monopolar spindles. In vitro, monastrol specifically inhibited the motility of the mitotic kinesin Eg5, a motor protein required for spindle bipolarity. All previously known small molecules that specifically affect the mitotic machinery target tubulin. Monastrol will therefore be a particularly useful tool for studying mitotic mechanisms.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Eg5, checkpoint, spindle, chromosome, Mitose, Chromosomenbande, Zellteilung, Mikrotubuli, Kinesin
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ISO 690MAYER, Thomas U., Tarun M. KAPOOR, Stephen J. HAGGARTY, Randall W. KING, Stuart L. SCHREIBER, Timothy J. MITCHISON, 1999. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. In: Science. 1999, 286(5441), pp. 971-974. ISSN 0036-8075. Available under: doi: 10.1126/science.286.5441.971
BibTex
@article{Mayer1999Small-14056,
  year={1999},
  doi={10.1126/science.286.5441.971},
  title={Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen},
  number={5441},
  volume={286},
  issn={0036-8075},
  journal={Science},
  pages={971--974},
  author={Mayer, Thomas U. and Kapoor, Tarun M. and Haggarty, Stephen J. and King, Randall W. and Schreiber, Stuart L. and Mitchison, Timothy J.}
}
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