Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen

Mayer, Thomas U.; Kapoor, Tarun M.; Haggarty, Stephen J.; King, Randall W.; Schreiber, Stuart L.; Mitchison, Timothy J.

Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen

Dateien

mayer_140561.pdfGröße: 461.77 KBDownloads: 1557

Datum

1999

Autor:innen

Mayer, Thomas U.

Kapoor, Tarun M.

Haggarty, Stephen J.

King, Randall W.

Schreiber, Stuart L.

Mitchison, Timothy J.

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Science. 1999, 286(5441), pp. 971-974. ISSN 0036-8075. Available under: doi: 10.1126/science.286.5441.971

Zusammenfassung

Small molecules that perturb specific protein functions are valuable tools for dissecting complex processes in mammalian cells. A combination of two phenotype-based screens, one based on a specific posttranslational modification, the other visualizing microtubules and chromatin, was used to identify compounds that affect mitosis. One compound, here named monastrol, arrested mammalian cells in mitosis with monopolar spindles. In vitro, monastrol specifically inhibited the motility of the mitotic kinesin Eg5, a motor protein required for spindle bipolarity. All previously known small molecules that specifically affect the mitotic machinery target tubulin. Monastrol will therefore be a particularly useful tool for studying mitotic mechanisms.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Eg5, checkpoint, spindle, chromosome, Mitose, Chromosomenbande, Zellteilung, Mikrotubuli, Kinesin

Zitieren

ISO 690

MAYER, Thomas U., Tarun M. KAPOOR, Stephen J. HAGGARTY, Randall W. KING, Stuart L. SCHREIBER, Timothy J. MITCHISON, 1999. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. In: Science. 1999, 286(5441), pp. 971-974. ISSN 0036-8075. Available under: doi: 10.1126/science.286.5441.971

BibTex

@article{Mayer1999Small-14056,
  year={1999},
  doi={10.1126/science.286.5441.971},
  title={Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen},
  number={5441},
  volume={286},
  issn={0036-8075},
  journal={Science},
  pages={971--974},
  author={Mayer, Thomas U. and Kapoor, Tarun M. and Haggarty, Stephen J. and King, Randall W. and Schreiber, Stuart L. and Mitchison, Timothy J.}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/14056">
    <dc:creator>Schreiber, Stuart L.</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14056"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14056/2/mayer_140561.pdf"/>
    <dcterms:issued>1999</dcterms:issued>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-11T11:20:57Z</dcterms:available>
    <dc:contributor>Haggarty, Stephen J.</dc:contributor>
    <dc:creator>Haggarty, Stephen J.</dc:creator>
    <dc:creator>King, Randall W.</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14056/2/mayer_140561.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-11T11:20:57Z</dc:date>
    <dc:contributor>King, Randall W.</dc:contributor>
    <dc:creator>Mayer, Thomas U.</dc:creator>
    <dc:contributor>Schreiber, Stuart L.</dc:contributor>
    <dcterms:title>Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen</dcterms:title>
    <dc:contributor>Mitchison, Timothy J.</dc:contributor>
    <dc:contributor>Mayer, Thomas U.</dc:contributor>
    <dc:creator>Mitchison, Timothy J.</dc:creator>
    <dcterms:bibliographicCitation>Science ; 286 (1999),  5441. - S. 971-974</dcterms:bibliographicCitation>
    <dc:creator>Kapoor, Tarun M.</dc:creator>
    <dc:contributor>Kapoor, Tarun M.</dc:contributor>
    <dcterms:abstract xml:lang="eng">Small molecules that perturb specific protein functions are valuable tools for dissecting complex processes in mammalian cells. A combination of two phenotype-based screens, one based on a specific posttranslational modification, the other visualizing microtubules and chromatin, was used to identify compounds that affect mitosis. One compound, here named monastrol, arrested mammalian cells in mitosis with monopolar spindles. In vitro, monastrol specifically inhibited the motility of the mitotic kinesin Eg5, a motor protein required for spindle bipolarity. All previously known small molecules that specifically affect the mitotic machinery target tubulin. Monastrol will therefore be a particularly useful tool for studying mitotic mechanisms.</dcterms:abstract>
    <dc:rights>terms-of-use</dc:rights>
    <dc:language>eng</dc:language>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Nein