Publikation:

Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus

Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2001

Autor:innen

Ivens, Andreas
Szadkowski, Halina
Wilmanns, Matthias
Kirschner, Kasper

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
DOI (zitierfähiger Link)
https://doi.org/10.1046/j.1432-1327.2001.02102.x
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung

Sammlungen

Biologie: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

European Journal of Biochemistry. 2001, 268(8), pp. 2246-2252. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1046/j.1432-1327.2001.02102.x

Zusammenfassung

Anthranilate phosphoribosyltransferase (TrpD; EC 2.4.2.18) from the hyperthermophilic archaeon Sulfolobus solfataricus (ssTrpD) was expressed in Escherichia coli, purified and crystallized. Analytical gel permeation chromatography revealed a homodimeric composition of the enzyme. The steady‐state kinetic characteristics suggest tight binding of the substrate anthranilic acid and efficient catalysis at the physiological growth temperature of S. solfataricus. Crystals of ssTrpD diffract to better than 2.6 Å resolution and preliminary X‐ray characterization was carried out. The crystals are suitable for structure determination.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

anthranilate phosphoribosyltransferase; protein crystallization; Sulfolobus solfataricus; tryptophan biosynthesis

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690IVENS, Andreas, Olga MAYANS, Halina SZADKOWSKI, Matthias WILMANNS, Kasper KIRSCHNER, 2001. Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus. In: European Journal of Biochemistry. 2001, 268(8), pp. 2246-2252. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1046/j.1432-1327.2001.02102.x
BibTex
@article{Ivens2001-12-20Purif-42021,
  year={2001},
  doi={10.1046/j.1432-1327.2001.02102.x},
  title={Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus},
  number={8},
  volume={268},
  issn={0014-2956},
  journal={European Journal of Biochemistry},
  pages={2246--2252},
  author={Ivens, Andreas and Mayans, Olga and Szadkowski, Halina and Wilmanns, Matthias and Kirschner, Kasper}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/42021">
    <dc:creator>Mayans, Olga</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Kirschner, Kasper</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:title>Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus</dcterms:title>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/42021"/>
    <dc:contributor>Wilmanns, Matthias</dc:contributor>
    <dc:language>eng</dc:language>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Ivens, Andreas</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Mayans, Olga</dc:contributor>
    <dc:creator>Kirschner, Kasper</dc:creator>
    <dc:creator>Ivens, Andreas</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-04-12T07:29:15Z</dcterms:available>
    <dc:contributor>Szadkowski, Halina</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>2001-12-20</dcterms:issued>
    <dc:creator>Wilmanns, Matthias</dc:creator>
    <dc:creator>Szadkowski, Halina</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-04-12T07:29:15Z</dc:date>
    <dcterms:abstract xml:lang="eng">Anthranilate phosphoribosyltransferase (TrpD; EC 2.4.2.18) from the hyperthermophilic archaeon Sulfolobus solfataricus (ssTrpD) was expressed in Escherichia coli, purified and crystallized. Analytical gel permeation chromatography revealed a homodimeric composition of the enzyme. The steady‐state kinetic characteristics suggest tight binding of the substrate anthranilic acid and efficient catalysis at the physiological growth temperature of S. solfataricus. Crystals of ssTrpD diffract to better than 2.6 Å resolution and preliminary X‐ray characterization was carried out. The crystals are suitable for structure determination.</dcterms:abstract>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen