Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus

Ivens, Andreas; Mayans, Olga; Szadkowski, Halina; Wilmanns, Matthias; Kirschner, Kasper

Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2001

Autor:innen

Ivens, Andreas

Mayans, Olga

Szadkowski, Halina

Wilmanns, Matthias

Kirschner, Kasper

DOI (zitierfähiger Link)

https://doi.org/10.1046/j.1432-1327.2001.02102.x

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

European Journal of Biochemistry. 2001, 268(8), pp. 2246-2252. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1046/j.1432-1327.2001.02102.x

Zusammenfassung

Anthranilate phosphoribosyltransferase (TrpD; EC 2.4.2.18) from the hyperthermophilic archaeon Sulfolobus solfataricus (ssTrpD) was expressed in Escherichia coli, purified and crystallized. Analytical gel permeation chromatography revealed a homodimeric composition of the enzyme. The steady‐state kinetic characteristics suggest tight binding of the substrate anthranilic acid and efficient catalysis at the physiological growth temperature of S. solfataricus. Crystals of ssTrpD diffract to better than 2.6 Å resolution and preliminary X‐ray characterization was carried out. The crystals are suitable for structure determination.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

anthranilate phosphoribosyltransferase; protein crystallization; Sulfolobus solfataricus; tryptophan biosynthesis

Zitieren

ISO 690

IVENS, Andreas, Olga MAYANS, Halina SZADKOWSKI, Matthias WILMANNS, Kasper KIRSCHNER, 2001. Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus. In: European Journal of Biochemistry. 2001, 268(8), pp. 2246-2252. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1046/j.1432-1327.2001.02102.x

BibTex

@article{Ivens2001-12-20Purif-42021,
  year={2001},
  doi={10.1046/j.1432-1327.2001.02102.x},
  title={Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus},
  number={8},
  volume={268},
  issn={0014-2956},
  journal={European Journal of Biochemistry},
  pages={2246--2252},
  author={Ivens, Andreas and Mayans, Olga and Szadkowski, Halina and Wilmanns, Matthias and Kirschner, Kasper}
}

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