A mass spectrometry-based hybrid method for structural modeling of protein complexes

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2014
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Politis, Argyris
Hall, Zoe
Hernández, Helena
Leitner, Alexander
Walzthoeni, Thomas
Robinson, Carol V.
Aebersold, Ruedi
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Zusammenfassung

We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.

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570 Biowissenschaften, Biologie
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ISO 690POLITIS, Argyris, Florian STENGEL, Zoe HALL, Helena HERNÁNDEZ, Alexander LEITNER, Thomas WALZTHOENI, Carol V. ROBINSON, Ruedi AEBERSOLD, 2014. A mass spectrometry-based hybrid method for structural modeling of protein complexes. In: Nature Methods. 2014, 11(4), pp. 403-406. ISSN 1548-7091. eISSN 1548-7105. Available under: doi: 10.1038/nmeth.2841
BibTex
@article{Politis2014spect-33030,
  year={2014},
  doi={10.1038/nmeth.2841},
  title={A mass spectrometry-based hybrid method for structural modeling of protein complexes},
  number={4},
  volume={11},
  issn={1548-7091},
  journal={Nature Methods},
  pages={403--406},
  author={Politis, Argyris and Stengel, Florian and Hall, Zoe and Hernández, Helena and Leitner, Alexander and Walzthoeni, Thomas and Robinson, Carol V. and Aebersold, Ruedi}
}
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