Plastid proteome prediction for diatoms and other algae with secondary plastids of the red lineage

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2015
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Rocap, Gabrielle
Armbrust, E. Virginia
Mock, Thomas
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The plastids of ecologically and economically important algae from phyla such as stramenopiles, dinoflagellates and cryptophytes were acquired via a secondary endosymbiosis and are surrounded by three or four membranes. Nuclear-encoded plastid-localized proteins contain N-terminal bipartite targeting peptides with the conserved amino acid sequence motif ‘ASAFAP’. Here we identify the plastid proteomes of two diatoms, Thalassiosira pseudonana and Phaeodactylum tricornutum, using a customized prediction tool (ASAFind) that identifies nuclear-encoded plastid proteins in algae with secondary plastids of the red lineage based on the output of SignalP and the identification of conserved ‘ASAFAP’ motifs and transit peptides. We tested ASAFind against a large reference dataset of diatom proteins with experimentally confirmed subcellular localization and found that the tool accurately identified plastid-localized proteins with both high sensitivity and high specificity. To identify nucleus-encoded plastid proteins of T. pseudonana and P. tricornutum we generated optimized sets of gene models for both whole genomes, to increase the percentage of full-length proteins compared with previous assembly model sets. ASAFind applied to these optimized sets revealed that about 8% of the proteins encoded in their nuclear genomes were predicted to be plastid localized and therefore represent the putative plastid proteomes of these algae.

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570 Biowissenschaften, Biologie
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Thalassiosira pseudonana, Phaeodactylum tricornutum, chloroplast, proteome, prediction, technical advance
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ISO 690GRUBER, Ansgar, Gabrielle ROCAP, Peter G. KROTH, E. Virginia ARMBRUST, Thomas MOCK, 2015. Plastid proteome prediction for diatoms and other algae with secondary plastids of the red lineage. In: The Plant Journal. 2015, 81(3), pp. 519-528. ISSN 0960-7412. eISSN 1365-313X. Available under: doi: 10.1111/tpj.12734
BibTex
@article{Gruber2015Plast-30684,
  year={2015},
  doi={10.1111/tpj.12734},
  title={Plastid proteome prediction for diatoms and other algae with secondary plastids of the red lineage},
  number={3},
  volume={81},
  issn={0960-7412},
  journal={The Plant Journal},
  pages={519--528},
  author={Gruber, Ansgar and Rocap, Gabrielle and Kroth, Peter G. and Armbrust, E. Virginia and Mock, Thomas}
}
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