Molecular techniques and their limitations shape our view of the holobiont

Kurzanzeige
dc.contributor.authorCooke, Ira
dc.contributor.authorMead, Oliver
dc.contributor.authorWhalen, Casey
dc.contributor.authorBoote, Chloë
dc.contributor.authorMoya, Aurelie
dc.contributor.authorYing, Hua
dc.contributor.authorRobbins, Steven
dc.contributor.authorStrugnell, Jan
dc.contributor.authorDarling, Aaron
dc.contributor.authorVoolstra, Christian R.
dc.date.accessioned2019-07-30T12:17:11Z
dc.date.available2019-07-30T12:17:11Z
dc.date.issued2019-12
dc.description.abstractIt is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA gene amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1016/j.zool.2019.125695eng
dc.identifier.pmid31759226
dc.identifier.ppn1685435394
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/46560
dc.language.isoengeng
dc.rightsterms-of-use
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/
dc.subjectHolobiont, Imaging, Metagenomic sequencing, Evolutionary inference, Model system, Multi-omicseng
dc.subject.ddc570eng
dc.titleMolecular techniques and their limitations shape our view of the holobionteng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
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  doi={10.1016/j.zool.2019.125695},
  title={Molecular techniques and their limitations shape our view of the holobiont},
  volume={137},
  issn={0944-2006},
  journal={Zoology},
  author={Cooke, Ira and Mead, Oliver and Whalen, Casey and Boote, Chloë and Moya, Aurelie and Ying, Hua and Robbins, Steven and Strugnell, Jan and Darling, Aaron and Voolstra, Christian R.},
  note={Article Number: 125695}
}
kops.citation.iso690COOKE, Ira, Oliver MEAD, Casey WHALEN, Chloë BOOTE, Aurelie MOYA, Hua YING, Steven ROBBINS, Jan STRUGNELL, Aaron DARLING, Christian R. VOOLSTRA, 2019. Molecular techniques and their limitations shape our view of the holobiont. In: Zoology. 2019, 137, 125695. ISSN 0944-2006. eISSN 1873-2720. Available under: doi: 10.1016/j.zool.2019.125695deu
kops.citation.iso690COOKE, Ira, Oliver MEAD, Casey WHALEN, Chloë BOOTE, Aurelie MOYA, Hua YING, Steven ROBBINS, Jan STRUGNELL, Aaron DARLING, Christian R. VOOLSTRA, 2019. Molecular techniques and their limitations shape our view of the holobiont. In: Zoology. 2019, 137, 125695. ISSN 0944-2006. eISSN 1873-2720. Available under: doi: 10.1016/j.zool.2019.125695eng
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