Molecular techniques and their limitations shape our view of the holobiont

Cooke, Ira; Mead, Oliver; Whalen, Casey; Boote, Chloë; Moya, Aurelie; Ying, Hua; Robbins, Steven; Strugnell, Jan; Darling, Aaron; Voolstra, Christian R.

Molecular techniques and their limitations shape our view of the holobiont

Dateien

Cooke_2-sykukfc1bw6t5.pdfGröße: 302.56 KBDownloads: 92

Datum

2019

Autor:innen

Cooke, Ira

Mead, Oliver

Whalen, Casey

Boote, Chloë

Moya, Aurelie

Ying, Hua

Robbins, Steven

Strugnell, Jan

Darling, Aaron

Voolstra, Christian R.

et al.

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Zoology. 2019, 137, 125695. ISSN 0944-2006. eISSN 1873-2720. Available under: doi: 10.1016/j.zool.2019.125695

Zusammenfassung

It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA gene amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Holobiont, Imaging, Metagenomic sequencing, Evolutionary inference, Model system, Multi-omics

Zitieren

ISO 690

COOKE, Ira, Oliver MEAD, Casey WHALEN, Chloë BOOTE, Aurelie MOYA, Hua YING, Steven ROBBINS, Jan STRUGNELL, Aaron DARLING, Christian R. VOOLSTRA, 2019. Molecular techniques and their limitations shape our view of the holobiont. In: Zoology. 2019, 137, 125695. ISSN 0944-2006. eISSN 1873-2720. Available under: doi: 10.1016/j.zool.2019.125695

BibTex

@article{Cooke2019-12Molec-46560,
  year={2019},
  doi={10.1016/j.zool.2019.125695},
  title={Molecular techniques and their limitations shape our view of the holobiont},
  volume={137},
  issn={0944-2006},
  journal={Zoology},
  author={Cooke, Ira and Mead, Oliver and Whalen, Casey and Boote, Chloë and Moya, Aurelie and Ying, Hua and Robbins, Steven and Strugnell, Jan and Darling, Aaron and Voolstra, Christian R.},
  note={Article Number: 125695}
}

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Universitätsbibliographie

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