Molecular techniques and their limitations shape our view of the holobiont

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2019
Autor:innen
Cooke, Ira
Mead, Oliver
Whalen, Casey
Boote, Chloë
Ying, Hua
Robbins, Steven
Strugnell, Jan
Darling, Aaron
et al.
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Zoology. 2019, 137, 125695. ISSN 0944-2006. eISSN 1873-2720. Available under: doi: 10.1016/j.zool.2019.125695
Zusammenfassung

It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA gene amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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Holobiont, Imaging, Metagenomic sequencing, Evolutionary inference, Model system, Multi-omics
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ISO 690COOKE, Ira, Oliver MEAD, Casey WHALEN, Chloë BOOTE, Aurelie MOYA, Hua YING, Steven ROBBINS, Jan STRUGNELL, Aaron DARLING, Christian R. VOOLSTRA, 2019. Molecular techniques and their limitations shape our view of the holobiont. In: Zoology. 2019, 137, 125695. ISSN 0944-2006. eISSN 1873-2720. Available under: doi: 10.1016/j.zool.2019.125695
BibTex
@article{Cooke2019-12Molec-46560,
  year={2019},
  doi={10.1016/j.zool.2019.125695},
  title={Molecular techniques and their limitations shape our view of the holobiont},
  volume={137},
  issn={0944-2006},
  journal={Zoology},
  author={Cooke, Ira and Mead, Oliver and Whalen, Casey and Boote, Chloë and Moya, Aurelie and Ying, Hua and Robbins, Steven and Strugnell, Jan and Darling, Aaron and Voolstra, Christian R.},
  note={Article Number: 125695}
}
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