GAP-43 immunoreactivity and axon regeneration in retinal ganglion cells of the rat

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1994
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Schaden, Herbert
Bähr, Martin
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Journal of Neurobiology. 1994, 25(12), pp. 1570-1578. ISSN 0022-3034. eISSN 1097-4695. Available under: doi: 10.1002/neu.480251209
Zusammenfassung

Retinal ganglion cells (RGCs) in rats were retrogradely labeled with the fluorescent tracer Fluorogold (FG) and subjected to GAP-43 and c-JUN immunocytochemistry to identify those RGSs that are capable of regenerating an axon. After optic nerve section (ONS) and simultaneous application of FG to the nerve stump (group 1 experiments), GAP-43 immunoreactive RGCs (between 2 and 21 days after ONS) always represented a subfraction of both FG-labeled (i.e., surviving) RGCs and RGCs exhibiting c-JUN. GAP-43 immunoreactive RGCs represented 22% of RGCs normally present in rat retinae and 25% of surviving RGCs at 5 days after ONS but were reduced to 2% and 1%, which is 6% and 5% of survivors at 14 and 21 days, respectively. In animals that received a peripheral nerve (PN) graft after ONS (group 2 experiments), RGCs with regenerating axons were identified by FG application to the graft at 14 and 21 days. When examined at 21 and 28 days, all FG-labeled RGCs exhibited GAP-43 immunoreactivity, and FG/GAP-43-labeled RGCs were 3% and 2% of those resent in normal rat retinae. In relation to surviving. RGCs GAP-43 immunoreactive RGCs represented 10% at both time points. FG-/GAP-43 labeled RGCs also exhibited c-JUN, but c-JUN immunoreactive RGCs were at both time points at least twice as numerous a FG-/GAP-43-labeled RGCs. These data suggest that regenerating axons in PN grafts derive specifically from GAP-43 reexpressing RGCs. Appearance of GAP-43 immunoreactivity may therefore identify those RGCs that are capable of axonal regeneration or sprouting.

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570 Biowissenschaften, Biologie
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rat optic nerve lesion, peripheral nerve graft, axonal regeneration, GAP-43/c-JUN immunocytochemistry, retinal ganglion cell quantification
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ISO 690SCHADEN, Herbert, Claudia STÜRMER, Martin BÄHR, 1994. GAP-43 immunoreactivity and axon regeneration in retinal ganglion cells of the rat. In: Journal of Neurobiology. 1994, 25(12), pp. 1570-1578. ISSN 0022-3034. eISSN 1097-4695. Available under: doi: 10.1002/neu.480251209
BibTex
@article{Schaden1994GAP43-8175,
  year={1994},
  doi={10.1002/neu.480251209},
  title={GAP-43 immunoreactivity and axon regeneration in retinal ganglion cells of the rat},
  number={12},
  volume={25},
  issn={0022-3034},
  journal={Journal of Neurobiology},
  pages={1570--1578},
  author={Schaden, Herbert and Stürmer, Claudia and Bähr, Martin}
}
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