Polyelectrolyte-mediated protein adsorption :  Fluorescent protein binding to individual polyelectrolyte nanospheres

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2005
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Anikin, Kirill
Röcker, Carlheinz
Wiedenmann, Jörg
Ballauff, Matthias
Nienhaus, G. Ulrich
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The Journal of Physical Chemistry B. 2005, 109(12), pp. 5418-5420. ISSN 1520-6106. Available under: doi: 10.1021/jp0506282
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We have used confocal fluorescence microscopy with single molecule sensitivity to characterize uptake and release of fluorescent protein (mEosFP) molecules by individual spherical polyelectrolyte brush (SPB) nanoparticles that were immobilized on a glass surface. The SPB particles consisted of a solid core particle of 100 nm diameter onto which long polyelectrolyte chains were affixed. They could be loaded with up to 30 000 mEosFP molecules in a solvent of low ionic strength. The concentration dependence of protein loading can be described with a simple bimolecular binding model, characterized by an equilibrium dissociation coefficient of 0.5 μM. Essentially complete release of the bound proteins was observed after increasing the ionic strength by adding 250 mM NaCl to the solvent. Fluorescence emission spectra and time-resolved fluorescence intensity decays were measured on individual, mEosFP-loaded SPB nanoparticles, and also on the dissolved mEosFP before and after adsorption. These results indicate that the mEosFP molecules remained structurally intact in this procedure. Hence, the present investigation demonstrates unambiguously that polyelectrolyte-mediated protein adsorption onto SPB particles presents a viable process for protein immobilization.

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ISO 690ANIKIN, Kirill, Carlheinz RÖCKER, Alexander WITTEMANN, Jörg WIEDENMANN, Matthias BALLAUFF, G. Ulrich NIENHAUS, 2005. Polyelectrolyte-mediated protein adsorption :  Fluorescent protein binding to individual polyelectrolyte nanospheres. In: The Journal of Physical Chemistry B. 2005, 109(12), pp. 5418-5420. ISSN 1520-6106. Available under: doi: 10.1021/jp0506282
BibTex
@article{Anikin2005-03-31Polye-20208,
  year={2005},
  doi={10.1021/jp0506282},
  title={Polyelectrolyte-mediated protein adsorption :  Fluorescent protein binding to individual polyelectrolyte nanospheres},
  number={12},
  volume={109},
  issn={1520-6106},
  journal={The Journal of Physical Chemistry B},
  pages={5418--5420},
  author={Anikin, Kirill and Röcker, Carlheinz and Wittemann, Alexander and Wiedenmann, Jörg and Ballauff, Matthias and Nienhaus, G. Ulrich}
}
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