Identification of the epitope for anti-cystatin C antibodies

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2011
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Sladewska, Anna
Szymanska, Aneta
Kordalska, Marlena
Lewandowska, Agnieszka
Kołodziejczyk, Aleksandra S.
Czaplewska, Paulina
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Journal of Molecular Recognition. 2011, 24(4), pp. 687-699. ISSN 0952-3499. eISSN 1099-1352. Available under: doi: 10.1002/jmr.1100
Zusammenfassung

Human cystatin C (hCC), like many other amyloidogenic proteins, has been shown to form dimers by exchange of subdomains of the monomeric protein. Considering the model of hCC fibrillogenesis by propagated domain swapping, it seems possible that inhibition of this process should also suppress the entire process of dimerization and fibrillogenesis which leads to specific amyloidosis (hereditary cystatin C amyloid angiopathy (HCCAA)). It was reported that exogenous agents like monoclonal antibody against cystatin C are able to suppress formation of cystatin C dimers. In the effort to find a way of controlling the cystatin fibrillization process, the interactions between monoclonal antibody Cyst-13 and cystatin C were studied in detail. The present work describes the determination of the epitope of hCC to a monoclonal antibody raised against cystatin C, Cyst-13, by MALDI mass spectrometry, using proteolytic excision of the immune complex. The shortest epitope sequence was determined as hCC(107-114). Affinity studies of synthetic peptides revealed that the octapeptide with epitope sequence does not have binding ability to Cyst-13, whereas its longer counterpart, hCC(105–114), binds the studied antibody. The secondary structure of the peptides with epitope sequence was studied using circular dichroism and NMR spectroscopy.

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Fachgebiet (DDC)
540 Chemie
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cystatin C, monoclonal antibody, epitope, mass spectrometry
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ISO 690SLADEWSKA, Anna, Aneta SZYMANSKA, Marlena KORDALSKA, Agnieszka LEWANDOWSKA, Aleksandra S. KOŁODZIEJCZYK, Gabriela-Ioana PARASCHIV, Michael PRZYBYLSKI, Paulina CZAPLEWSKA, 2011. Identification of the epitope for anti-cystatin C antibodies. In: Journal of Molecular Recognition. 2011, 24(4), pp. 687-699. ISSN 0952-3499. eISSN 1099-1352. Available under: doi: 10.1002/jmr.1100
BibTex
@article{Sladewska2011-07Ident-471,
  year={2011},
  doi={10.1002/jmr.1100},
  title={Identification of the epitope for anti-cystatin C antibodies},
  number={4},
  volume={24},
  issn={0952-3499},
  journal={Journal of Molecular Recognition},
  pages={687--699},
  author={Sladewska, Anna and Szymanska, Aneta and Kordalska, Marlena and Lewandowska, Agnieszka and Kołodziejczyk, Aleksandra S. and Paraschiv, Gabriela-Ioana and Przybylski, Michael and Czaplewska, Paulina}
}
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