DNA Conjugation by the Staudinger Ligation : New Thymidine Analogues

Baccaro, Anna; Weisbrod, Samuel H.; Marx, Andreas

DNA Conjugation by the Staudinger Ligation : New Thymidine Analogues

Dateien

Baccaro_0-329947.pdfGröße: 700.94 KBDownloads: 344

Datum

2007

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Synthesis : journal of synthetic organic chemistry. 2007, 2007(13), pp. 1949-1954. ISSN 0039-7881. eISSN 1437-210X. Available under: doi: 10.1055/s-2007-983728

Zusammenfassung

Two novel modified 2′-deoxyuridine triphosphates carrying an azide functionality linked to the nucleobase were synthesized. For probing the sterical influence on enzymatic incorporation and Staudinger ligation, differently sized flexible linkers were chosen. Both nucleotides can completely replace natural thymidine in primer extension as well as polymerase chain reaction (PCR) using Pyrococcus woesei DNA polymerase. For PCR with larger gene fragments as template, however, the longer linker disturbs the DNA polymerase and yields less product. For azide-labeled primer extension products, subsequent conjugation of suitably functionalized phosphines via Staudinger ligation was achieved, for example for the conjugation of biotin as an affinity tag.

Fachgebiet (DDC)

540 Chemie

Zitieren

ISO 690

BACCARO, Anna, Samuel H. WEISBROD, Andreas MARX, 2007. DNA Conjugation by the Staudinger Ligation : New Thymidine Analogues. In: Synthesis : journal of synthetic organic chemistry. 2007, 2007(13), pp. 1949-1954. ISSN 0039-7881. eISSN 1437-210X. Available under: doi: 10.1055/s-2007-983728

BibTex

@article{Baccaro2007-07Conju-33838,
  year={2007},
  doi={10.1055/s-2007-983728},
  title={DNA Conjugation by the Staudinger Ligation : New Thymidine Analogues},
  number={13},
  volume={2007},
  issn={0039-7881},
  journal={Synthesis : journal of synthetic organic chemistry},
  pages={1949--1954},
  author={Baccaro, Anna and Weisbrod, Samuel H. and Marx, Andreas}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/33838">
    <dcterms:issued>2007-07</dcterms:issued>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:title>DNA Conjugation by the Staudinger Ligation : New Thymidine Analogues</dcterms:title>
    <dc:creator>Weisbrod, Samuel H.</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/33838"/>
    <dc:creator>Marx, Andreas</dc:creator>
    <dc:contributor>Weisbrod, Samuel H.</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/33838/1/Baccaro_0-329947.pdf"/>
    <dcterms:abstract xml:lang="eng">Two novel modified 2′-deoxyuridine triphosphates carrying an azide functionality linked to the nucleobase were synthesized. For probing the sterical influence on enzymatic incorporation and Staudinger ligation, differently sized flexible linkers were chosen. Both nucleotides can completely replace natural thymidine in primer extension as well as polymerase chain reaction (PCR) using Pyrococcus woesei DNA polymerase. For PCR with larger gene fragments as template, however, the longer linker disturbs the DNA polymerase and yields less product. For azide-labeled primer extension products, subsequent conjugation of suitably functionalized phosphines via Staudinger ligation was achieved, for example for the conjugation of biotin as an affinity tag.</dcterms:abstract>
    <dc:creator>Baccaro, Anna</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Baccaro, Anna</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-05-09T12:56:01Z</dcterms:available>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-05-09T12:56:01Z</dc:date>
    <dc:contributor>Marx, Andreas</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:language>eng</dc:language>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/33838/1/Baccaro_0-329947.pdf"/>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja