Growth Suppression Induced by Downregulation of E6-AP Expression in Human Papillomavirus-Positive Cancer Cell Lines Depends on p53

Hengstermann, Arnd; D'silva, Michael A.; Kuballa, Petric; Butz, Karin; Hoppe-Seyler, Felix; Scheffner, Martin

Growth Suppression Induced by Downregulation of E6-AP Expression in Human Papillomavirus-Positive Cancer Cell Lines Depends on p53

Dateien

Hengstermann_167680.pdfGröße: 269.05 KBDownloads: 366

Datum

2005

Autor:innen

Hengstermann, Arnd

D'silva, Michael A.

Kuballa, Petric

Butz, Karin

Hoppe-Seyler, Felix

Scheffner, Martin

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of Virology. 2005, 79(14), pp. 9296-9300. ISSN 0022-538X. Available under: doi: 10.1128/JVI.79.14.9296-9300.2005

Zusammenfassung

The ubiquitin-protein ligase E6-AP is utilized by the E6 oncoprotein of human papillomaviruses (HPVs) associated with cervical cancer to target the tumor suppressor p53 for degradation. Here, we report that downregulation of E6-AP expression by RNA interference results in both the accumulation of p53 and growth suppression of the HPV-positive cervical cancer cell lines HeLa and SiHa. In addition, HeLa cells, in which p53 expression was suppressed by RNA interference, are significantly less sensitive to the downregulation of E6-AP expression with respect to growth suppression than parental HeLa cells. These data indicate that the anti-growth-suppressive properties of E6-AP in HPV-positive cells depend on its ability to induce p53 degradation.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

HENGSTERMANN, Arnd, Michael A. D'SILVA, Petric KUBALLA, Karin BUTZ, Felix HOPPE-SEYLER, Martin SCHEFFNER, 2005. Growth Suppression Induced by Downregulation of E6-AP Expression in Human Papillomavirus-Positive Cancer Cell Lines Depends on p53. In: Journal of Virology. 2005, 79(14), pp. 9296-9300. ISSN 0022-538X. Available under: doi: 10.1128/JVI.79.14.9296-9300.2005

BibTex

@article{Hengstermann2005-07Growt-16768,
  year={2005},
  doi={10.1128/JVI.79.14.9296-9300.2005},
  title={Growth Suppression Induced by Downregulation of E6-AP Expression in Human Papillomavirus-Positive Cancer Cell Lines Depends on p53},
  number={14},
  volume={79},
  issn={0022-538X},
  journal={Journal of Virology},
  pages={9296--9300},
  author={Hengstermann, Arnd and D'silva, Michael A. and Kuballa, Petric and Butz, Karin and Hoppe-Seyler, Felix and Scheffner, Martin}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/16768">
    <dc:creator>Scheffner, Martin</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Hengstermann, Arnd</dc:creator>
    <dc:contributor>Scheffner, Martin</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-11-16T08:24:37Z</dcterms:available>
    <dc:contributor>Hengstermann, Arnd</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/16768"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/16768/2/Hengstermann_167680.pdf"/>
    <dcterms:title>Growth Suppression Induced by Downregulation of E6-AP Expression in Human Papillomavirus-Positive Cancer Cell Lines Depends on p53</dcterms:title>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-11-16T08:24:37Z</dc:date>
    <dc:creator>Hoppe-Seyler, Felix</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/16768/2/Hengstermann_167680.pdf"/>
    <dcterms:issued>2005-07</dcterms:issued>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:language>eng</dc:language>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Butz, Karin</dc:creator>
    <dcterms:abstract xml:lang="eng">The ubiquitin-protein ligase E6-AP is utilized by the E6 oncoprotein of human papillomaviruses (HPVs) associated with cervical cancer to target the tumor suppressor p53 for degradation. Here, we report that downregulation of E6-AP expression by RNA interference results in both the accumulation of p53 and growth suppression of the HPV-positive cervical cancer cell lines HeLa and SiHa. In addition, HeLa cells, in which p53 expression was suppressed by RNA interference, are significantly less sensitive to the downregulation of E6-AP expression with respect to growth suppression than parental HeLa cells. These data indicate that the anti-growth-suppressive properties of E6-AP in HPV-positive cells depend on its ability to induce p53 degradation.</dcterms:abstract>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Butz, Karin</dc:contributor>
    <dc:creator>Kuballa, Petric</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>D'silva, Michael A.</dc:contributor>
    <dcterms:bibliographicCitation>Publ. in: Journal of Virology ; 79 (2005), 14. - S. 9296-9300</dcterms:bibliographicCitation>
    <dc:contributor>Hoppe-Seyler, Felix</dc:contributor>
    <dc:creator>D'silva, Michael A.</dc:creator>
    <dc:contributor>Kuballa, Petric</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja