Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane

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2019
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Cells. 2019, 8(11), 1388. eISSN 2073-4409. Available under: doi: 10.3390/cells8111388
Zusammenfassung

T cell activation is immediately followed by internalization of the T cell receptor (TCR). TCR endocytosis is required for T cell activation, but the mechanisms supporting removal of TCR from the cell surface remain incompletely understood. Here we report that TCR endocytosis is linked to the clathrin-independent carrier (CLIC) and GPI-enriched endocytic compartments (GEEC) endocytic pathway. We show that unlike the canonical clathrin cargo transferrin or the adaptor protein Lat, internalized TCR accumulates in tubules shaped by the small GTPase Cdc42 and the Bin/amphiphysin/Rvs (BAR) domain containing protein GRAF1 in T cells. Preventing GRAF1-positive tubules to mature into endocytic vesicles by expressing a constitutively active Cdc42 impairs the endocytosis of TCR, while having no consequence on the uptake of transferrin. Together, our data reveal a link between TCR internalization and the CLIC/GEEC endocytic route supported by Cdc42 and GRAF1.

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570 Biowissenschaften, Biologie
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T cell receptor; clathrin-independent endocytosis; CLIC; Cdc42; GRAF1
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Zitieren
ISO 690ROSSATTI, Pascal, Luca ZIEGLER, Richard SCHREGLE, Verena M. BETZLER, Manuela ECKER, Jérémie ROSSY, 2019. Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane. In: Cells. 2019, 8(11), 1388. eISSN 2073-4409. Available under: doi: 10.3390/cells8111388
BibTex
@article{Rossatti2019-11-04Cdc42-47519,
  year={2019},
  doi={10.3390/cells8111388},
  title={Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane},
  number={11},
  volume={8},
  journal={Cells},
  author={Rossatti, Pascal and Ziegler, Luca and Schregle, Richard and Betzler, Verena M. and Ecker, Manuela and Rossy, Jérémie},
  note={Article Number: 1388}
}
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